Busquets Sílvia, Deans Chris, Figueras Maite, Moore-Carrasco Rodrigo, López-Soriano Francisco J, Fearon Kenneth C H, Argilés Josep M
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Barcelona, Spain.
Clin Nutr. 2007 Oct;26(5):614-8. doi: 10.1016/j.clnu.2007.06.005. Epub 2007 Aug 3.
BACKGROUND & AIMS: Previous studies of our research group have shown that apoptosis is present in skeletal muscle of tumour-bearing animals subject to cachexia. For this reason we decided to investigate the apoptosis in skeletal muscle of cancer patients.
In the present study, muscle biopsies from weight-losing patients with upper gastro-intestinal cancer showed a significant increase in muscle DNA fragmentation (three-fold), as compared with control subjects. The increase in DNA laddering was associated with an increase in poly(ADP-ribose) polymerase (PARP) cleavage (four-fold) as measured by western blotting. These two events indicate the presence of muscle apoptosis. These changes were associated with a decrease in MyoD protein content, suggesting important alterations in skeletal muscle physiology.
The results presented therefore confirm that apoptosis is also present in human subjects undergoing cancer cachexia.
我们研究小组之前的研究表明，在患有恶病质的荷瘤动物的骨骼肌中存在细胞凋亡。因此，我们决定研究癌症患者骨骼肌中的细胞凋亡情况。
在本研究中，与对照组相比，来自体重减轻的上消化道癌症患者的肌肉活检显示肌肉DNA片段化显著增加（三倍）。通过蛋白质印迹法检测，DNA梯状条带的增加与聚（ADP - 核糖）聚合酶（PARP）裂解的增加（四倍）相关。这两个事件表明存在肌肉细胞凋亡。这些变化与肌分化抗原（MyoD）蛋白含量的降低相关，提示骨骼肌生理发生了重要改变。
因此，所呈现的结果证实细胞凋亡也存在于患有癌症恶病质的人类受试者中。
