中国小型猪中猪内源性逆转录病毒的大规模调查。

Large-scale survey of porcine endogenous retrovirus in Chinese miniature pigs.

作者信息

Wu Jianmin, Ma Yuyuan, Lv Maomin, Yang Yubiao, Guo Yanru, Yu Xiuling, Tian Kegong, Zhang Jingang

机构信息

Department of Blood Biopharmaceuticals and Biotechnology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.

出版信息

Comp Immunol Microbiol Infect Dis. 2008 Jul;31(4):367-71. doi: 10.1016/j.cimid.2007.06.004. Epub 2007 Aug 8.

DOI:10.1016/j.cimid.2007.06.004

PMID:17689611

Abstract

We conducted a large-scale survey on the existence and expression status of porcine endogenous retrovirus (PERV) in seven breeds of Chinese miniature pigs. Genotyping of PERV was examined by PCR using type-specific primers according to the env genotyping method. The presence and expression status of viral gag, pol and env genes were further analyzed in Wuzhishan pigs (WZSP) and Bama minipigs (BMP). The results showed that PERV existed in all 348 genomic DNA samples. The genotype distribution was subtype A-74.43%, subtype B-95.40% and subtype C-30.46%. No expression of subtype C was found in WZSP and BMP. This research obtained an adequate level of information on the molecular epidemiology of PERV in China. The results indicated that it is possible to monitor pig herds for individuals with the lowest PERV prevalence, especially lacking PERV-C.

摘要

我们对七种中国小型猪品种中猪内源性逆转录病毒（PERV）的存在和表达状况进行了大规模调查。根据env基因分型方法，使用型特异性引物通过PCR对PERV进行基因分型。进一步分析了五指山猪（WZSP）和巴马小型猪（BMP）中病毒gag、pol和env基因的存在和表达状况。结果显示，在所有348份基因组DNA样本中均存在PERV。基因型分布为A亚型-74.43%，B亚型-95.40%，C亚型-30.46%。在WZSP和BMP中未发现C亚型的表达。本研究获得了关于中国PERV分子流行病学的足够信息水平。结果表明，有可能对猪群进行监测，以找出PERV流行率最低的个体，尤其是缺乏PERV-C的个体。