Naegele R J, Lipari J, Chakkalakal D, Strates B, McGuire M
Orthopaedic Research Laboratory, V.A. Medical Center, Omaha, NE 68105.
Cancer Biochem Biophys. 1991 Aug;12(2):95-101.
In vitro electrical stimulation of human osteosarcoma-derived cells resulted in increased cell adherence and current directed cell migration. We have developed an electrical exposure system in which two steel electrodes imbedded in media-based agar, poured in a standard culture dish, are used to apply electric field signals to cells in culture without ion contamination from the electrodes. The cells were exposed to a 100 Hz pulsed DC electric signal at peak field strengths of 1, 10, 100, and 625 mV/cm in the culture media. The data showed no change in cell adherence at 1 and 10 mV/cm, an increase in adherence at 100 mV/cm, and a decrease in both adherence and cell proliferation at 625 mV/cm. Electric field stimulation in vivo has been found useful in accelerating the healing of fractures and non-unions, and the repair of surgical and cancer-related skeletal defects.
对人骨肉瘤衍生细胞进行体外电刺激会导致细胞黏附增加以及电流引导的细胞迁移。我们开发了一种电暴露系统，其中嵌入基于培养基的琼脂并倒入标准培养皿中的两个钢电极用于向培养中的细胞施加电场信号，而不会受到来自电极的离子污染。在培养基中，细胞暴露于峰值场强为1、10、100和625 mV/cm的100 Hz脉冲直流电信号下。数据显示，在1和10 mV/cm时细胞黏附没有变化，在100 mV/cm时黏附增加，而在625 mV/cm时黏附和细胞增殖均下降。已发现体内电场刺激有助于加速骨折和骨不连的愈合以及手术和癌症相关骨骼缺损的修复。
