Ono O, Ando J, Kamiya A, Kuboki Y, Yasuda H
Department of Cardiovascular Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Cell Struct Funct. 1991 Oct;16(5):365-74. doi: 10.1247/csf.16.365.
Cultured vascular endothelial cells were exposed to fluid shear stress by means of a rotary-disc shear-loading device, and the physiological effects of the conditioned medium (CM) and the homogenate (HM) of the cells on migration, adhesion and growth of endothelial cells (EC) or smooth muscle cells (SMC) were studied. Effects of shear stress on the production and secretion of collagen, one of the extracellular matrices of EC, were also studied. CM stimulated the adhesion and growth of SMC, but not of EC themselves. The ability to stimulate SMC adhesion and growth was similar in CM obtained from the static and shear-loaded cells. HM of the shear-loaded EC stimulated SMC migration. Further, HM of the shear-loaded EC contained increased amounts of collagen compared with the static EC. These results suggest that: 1) EC produce and secrete accelerators for the adhesion and growth of SMC, 2) EC react to the physical stimulus of fluid shear stress to produce stimulators of SMC migration, and 3) EC produce collagen, the production of which is enhanced by fluid shear stress.
通过转盘剪切加载装置使培养的血管内皮细胞暴露于流体剪切应力下，研究了细胞条件培养基（CM）和匀浆（HM）对内皮细胞（EC）或平滑肌细胞（SMC）迁移、黏附和生长的生理影响。还研究了剪切应力对EC细胞外基质之一胶原蛋白产生和分泌的影响。CM刺激了SMC的黏附和生长，但对EC本身没有刺激作用。从静态细胞和经剪切加载的细胞获得的CM刺激SMC黏附和生长的能力相似。经剪切加载的EC的HM刺激了SMC的迁移。此外，与静态EC相比，经剪切加载的EC的HM中胶原蛋白含量增加。这些结果表明：1）EC产生并分泌促进SMC黏附和生长的因子；2）EC对流体剪切应力的物理刺激作出反应，产生SMC迁移的刺激因子；3）EC产生胶原蛋白，流体剪切应力可增强其产生。
