Wada-Isoe K, Michio K, Imamura K, Nakaso K, Kusumi M, Kowa H, Nakashima K
Department of Neurology, Faculty of Medicine, Institute of Neurological Sciences, Tottori University, Yonago, Japan.
J Neural Transm (Vienna). 2007;114(12):1579-83. doi: 10.1007/s00702-007-0794-5. Epub 2007 Aug 10.
Dementia with Lewy bodies (DLB) is the second most common senile degenerative dementia after Alzheimer's disease (AD). The presentation of overlapping symptoms between these two disorders leads to difficulties in the determination of clinical entities. Serum samples were subjected to surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) analysis in order to identify a diagnostic marker for DLB. Four putative protein peaks (m/z 3,883, 4,964, 7,761 and 10,534) were differentially expressed in DLB patients compared to AD patients and control subjects. Receiver operating characteristics (ROC) analysis of a multivariate logistic model of the combination of three peaks (m/z 3,883, 7,761 and 10,534) exhibited the highest discriminatory ability of DLB subjects from non-DLB subjects with a sensitivity of 83.3%, a specificity of 95.8%, a positive predictive value of 90.9% and a negative predictive value of 92.0%. SELDI-TOF MS profiling, therefore, has revealed a serum signature with high diagnostic potential for DLB.
路易体痴呆（DLB）是仅次于阿尔茨海默病（AD）的第二常见的老年性退行性痴呆。这两种疾病之间重叠症状的表现导致临床实体的确定存在困难。为了鉴定DLB的诊断标志物，对血清样本进行了表面增强激光解吸/电离飞行时间质谱（SELDI-TOF MS）分析。与AD患者和对照受试者相比，DLB患者中有四个假定的蛋白峰（m/z 3,883、4,964、7,761和10,534）差异表达。对三个峰（m/z 3,883、7,761和10,534）组合的多变量逻辑模型进行的受试者工作特征（ROC）分析显示，区分DLB受试者与非DLB受试者的能力最高，灵敏度为83.3%，特异性为95.8%，阳性预测值为90.9%，阴性预测值为92.0%。因此，SELDI-TOF MS分析揭示了一种对DLB具有高诊断潜力的血清特征。
