Villar-Garea Ana, Griese Matthias, Imhof Axel
Histone modifications group, Adolf-Butenandt Institute, Ludwig-Maximilians University of Munich, Schillerstr 44, 80336 Munich, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):105-14. doi: 10.1016/j.jchromb.2006.09.017. Epub 2006 Nov 9.
Systems analysis of body fluids by mass spectrometry (MS) is an upcoming field of biomarker research. This approach is extremely attractive because it does not require biomarker candidates and the sample preparation is simple. However, during the development of the technique strong critical comments were made on the poor reproducibility, the special characteristics of blood as a source of peptides and on the frequent non-adequate statistical analysis of the data. Here we discuss the efforts made in the last few years to develop suitable protocols, which could lead to biomarker discovery from body fluids by mass spectrometry. Our review focuses on the systems analysis of non-digested blood serum or plasma samples by MALDI-TOF and SELDI-TOF.
通过质谱（MS）对体液进行系统分析是生物标志物研究中一个新兴的领域。这种方法极具吸引力，因为它不需要候选生物标志物，且样品制备简单。然而，在该技术的发展过程中，人们对其重现性差、血液作为肽来源的特殊性质以及数据统计分析常常不足等方面提出了强烈的批评意见。在此，我们讨论了过去几年为开发合适的方案所做的努力，这些努力可能会通过质谱从体液中发现生物标志物。我们的综述重点关注通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和表面增强激光解吸电离飞行时间质谱（SELDI-TOF）对未消化的血清或血浆样品进行系统分析。
