Guo Q X, Chau R M, Yang S Z, Jen L S
Department of Anatomy, Chinese University of Hong Kong.
Brain Res Dev Brain Res. 1991 Oct 21;62(2):177-87. doi: 10.1016/0165-3806(91)90165-f.
Retinas from embryonic day 14 (E14) Sprague-Dawley rats were transplanted to the tectum of newborn (P0) recipient rats, and the distribution pattern of choline acetyltransferase immunoreactivity (ChAT-I) in developing transplants was studied and compared with those observed in the retinas of normal developing rats. In normal retinas, ChAT-I cells were first identified in restricted regions in the ganglion cell layer (GCL) at P4, but were found to cover the entire GCL by P6. A second population of ChAT-I cells was detected in the inner nuclear layer (INL) at P8, and they were observed in most parts of the INL on P10 when two immunoreactive sublaminae began to appear in the inner plexiform layer (IPL). The adult pattern of having two distinct populations of ChAT-I cells, organized in mirror symmetrical fashion in the inner retinal layers was basically established by P12. The time course of development and overall distribution pattern of ChAT-I cells in developing retinal transplants on the whole were very similar to those observed in normal retinas. The first identification of these cells and the establishment of their final distribution pattern were made at stages corresponding to P4 and P12 of normal developing retinas respectively. However, ChAT-I somata were located in the INL at a much earlier stage compared with their counterparts in the normal retina, and a transient population of immunoreactive cells with their processes extending to retinal layers other than the IPL was observed in some transplants from P6 to P10. These features were not observed in normal developing retinas. These results suggest that the development of cholinergic neurons, especially the expression of their characteristic antigen and their final distribution pattern is largely determined by programmes which are intrinsic to the original retinal tissue, despite some minor deviation or variation in the developmental process which may occur under certain abnormal conditions.
将胚胎第14天（E14）的斯普拉格-道利大鼠的视网膜移植到新生（P0）受体大鼠的视顶盖，研究发育中的移植视网膜中胆碱乙酰转移酶免疫反应性（ChAT-I）的分布模式，并与正常发育大鼠视网膜中的情况进行比较。在正常视网膜中，P4时在神经节细胞层（GCL）的特定区域首次发现ChAT-I细胞，但到P6时这些细胞覆盖了整个GCL。P8时在内核层（INL）检测到第二批ChAT-I细胞，P10时在内网层（IPL）开始出现两个免疫反应性亚层时，这些细胞出现在INL的大部分区域。到P12时，视网膜内层中以镜像对称方式组织的两种不同类型ChAT-I细胞的成年模式基本形成。发育中的视网膜移植中ChAT-I细胞的发育时间进程和整体分布模式总体上与正常视网膜中观察到的非常相似。这些细胞的首次识别及其最终分布模式的建立分别对应于正常发育视网膜的P4和P12阶段。然而，与正常视网膜中的对应细胞相比，ChAT-I胞体在更早阶段就位于INL中，并且在一些从P6到P10的移植中观察到一群短暂的免疫反应性细胞，其突起延伸到IPL以外的视网膜层。正常发育的视网膜中未观察到这些特征。这些结果表明，胆碱能神经元的发育，尤其是其特征性抗原的表达及其最终分布模式，在很大程度上由原始视网膜组织固有的程序决定，尽管在某些异常条件下发育过程中可能会出现一些轻微的偏差或变化。
