Development of gamma-aminobutyric acid-immunoreactive neurons in normal and intracranially transplanted retinas in rats.

Retinas from embryonic day 14 Sprague-Dawley rats were transplanted intracranially to the midbrain or cortex of newborn (P0) rats with right eyes enucleated at the time of transplantation and the gamma-aminobutyric acid (GABA) immunoreactivity in developing retinal transplants, host as well as normal retinas, was studied. The results showed that GABA-immunoreactive neurons were identified in retinas of normal and host rats from the day of birth (P0) onward and that their somata were distributed primarily in the inner half of the internal nuclear layer and in the ganglion cell layer. The adult pattern of GABA immunoreactivity was first observed at P16 when several immunoreactive sublaminae were clearly identifiable in the inner plexiform layer. In contrast, gamma-aminobutyric acid-immunoreactive somata could not be identified in retinal transplants until P4, with a significant reduction in the density and number of GABAergic neurons detected by P12. Moreover, only two immunoreactive sublaminae were observed in the inner plexiform layer in all transplants at P12, as well as in more mature stages. These results suggest that significant changes occurred in the GABA system of the transplanted retina, despite the fact that the overall pattern of organization of the GABAergic neurons and their processes in the retinal transplants was comparable to that of the normal retina.