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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Möck A, Renwrantz L

Zoologisches Institut der Universität Hamburg, FRG.

Comp Biochem Physiol B. 1991;99(3):699-707. doi: 10.1016/0305-0491(91)90357-j.

A lectin was isolated from an extract of Branchiostoma lanceolatum by affinity chromatography using an asialo-A-peptone-cellulose column. 2 The lectin is a glycoprotein with a carbohydrate content of 2.7%. The mol. wt is 392,000 +/- 28,000. Two subunits of identical size (183,000 +/- 3000) are linked by non-covalent bonds. 3. The lectin agglutinates a variety of erythrocytes including human A, B, O red blood cells as well as human lymphocytes. 4. Hemagglutination activity is inhibited best by N,N',N"-triacetylchitotriose, followed by N,N'-diacetylchitobiose, which is half as inhibitory. 5. Lectin activity is constant between pH 5 and 10. Divalent cations are not required for binding reactions. Activity is totally destroyed by heating to 60 degrees C for 30 min. 6. The lectin is precipitated from the extract by 30-40% ammonium sulfate saturation.

通过使用去唾液酸A-蛋白胨纤维素柱的亲和色谱法，从文昌鱼提取物中分离出一种凝集素。2. 该凝集素是一种糖蛋白，碳水化合物含量为2.7%。分子量为392,000±28,000。两个大小相同的亚基（183,000±3000）通过非共价键相连。3. 该凝集素能凝集多种红细胞，包括人类A、B、O型红细胞以及人类淋巴细胞。4. 血凝活性受N,N',N"-三乙酰壳三糖抑制效果最佳，其次是N,N'-二乙酰壳二糖，其抑制作用为前者的一半。5. 凝集素活性在pH 5至10之间保持恒定。结合反应不需要二价阳离子。加热至60摄氏度30分钟会使活性完全丧失。6. 通过30%-40%硫酸铵饱和度从提取物中沉淀出凝集素。

Möck A, Renwrantz L

Zoologisches Institut der Universität Hamburg, FRG.

Comp Biochem Physiol B. 1991;99(3):699-707. doi: 10.1016/0305-0491(91)90357-j.

A lectin was isolated from an extract of Branchiostoma lanceolatum by affinity chromatography using an asialo-A-peptone-cellulose column. 2 The lectin is a glycoprotein with a carbohydrate content of 2.7%. The mol. wt is 392,000 +/- 28,000. Two subunits of identical size (183,000 +/- 3000) are linked by non-covalent bonds. 3. The lectin agglutinates a variety of erythrocytes including human A, B, O red blood cells as well as human lymphocytes. 4. Hemagglutination activity is inhibited best by N,N',N"-triacetylchitotriose, followed by N,N'-diacetylchitobiose, which is half as inhibitory. 5. Lectin activity is constant between pH 5 and 10. Divalent cations are not required for binding reactions. Activity is totally destroyed by heating to 60 degrees C for 30 min. 6. The lectin is precipitated from the extract by 30-40% ammonium sulfate saturation.

通过使用去唾液酸A-蛋白胨纤维素柱的亲和色谱法，从文昌鱼提取物中分离出一种凝集素。2. 该凝集素是一种糖蛋白，碳水化合物含量为2.7%。分子量为392,000±28,000。两个大小相同的亚基（183,000±3000）通过非共价键相连。3. 该凝集素能凝集多种红细胞，包括人类A、B、O型红细胞以及人类淋巴细胞。4. 血凝活性受N,N',N"-三乙酰壳三糖抑制效果最佳，其次是N,N'-二乙酰壳二糖，其抑制作用为前者的一半。5. 凝集素活性在pH 5至10之间保持恒定。结合反应不需要二价阳离子。加热至60摄氏度30分钟会使活性完全丧失。6. 通过30%-40%硫酸铵饱和度从提取物中沉淀出凝集素。