人类无功能神经内分泌肿瘤中的生长抑素受体亚型以及生长抑素类似物SOM230对NCI-H727细胞系细胞增殖的影响。

Somatostatin receptor subtypes in human non-functioning neuroendocrine tumors and effects of somatostatin analogue SOM230 on cell proliferation in cell line NCI-H727.

作者信息

Ono Katsuhiko, Suzuki Takashi, Miki Yasuhiro, Taniyama Yusuke, Nakamura Yasuhiro, Noda Yutaka, Watanabe Mika, Sasano Hironobu

机构信息

Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.

出版信息

Anticancer Res. 2007 Jul-Aug;27(4B):2231-9.

PMID:17695508

Abstract

Well-differentiated neuroendocrine tumors (NT) expresses somatostatin receptors (sstr). A composition of sstr subtype determines biological behavior. We therefore evaluated their immunolocalization in 71 NT cases using immunohistochemistry. Sstr immunoreactivity was demonstrated as follows: sstr1 39.4% of all cases, sstr2A 90.1%, sstr2B 39.4%, sstr3 50.7% and sstr5 76.1%. Based on these findings, the effects of the recently developed sstr subtype-specific somatostatin analogue SOM230 on the NT cell line NCI-H727 were examined. SOM230 significantly reduced cell proliferation while the conventional analogue SMS201-995 did not. Therefore, SOM230 has advantages in controlling the cell proliferation of these tumors but the status of sstr subtypes should be determined, possibly using immunohistochemistry, in order to confer its maximum benefits.

摘要

高分化神经内分泌肿瘤（NT）表达生长抑素受体（sstr）。sstr亚型的组成决定生物学行为。因此，我们使用免疫组织化学评估了71例NT病例中它们的免疫定位。sstr免疫反应性表现如下：sstr1在所有病例中占39.4%，sstr2A占90.1%，sstr2B占39.4%，sstr3占50.7%，sstr5占76.1%。基于这些发现，研究了最近开发的sstr亚型特异性生长抑素类似物SOM230对NT细胞系NCI-H727的影响。SOM230显著降低细胞增殖，而传统类似物SMS201-995则没有。因此，SOM230在控制这些肿瘤的细胞增殖方面具有优势，但为了发挥其最大效益，可能需要使用免疫组织化学来确定sstr亚型的状态。