Biggiogera Marco, Cisterna Barbara, Spedito Alessandro, Vecchio Lorella, Malatesta Manuela
Dipartimento di Biologia Animale, Laboratorio di Biologia Cellulare e Neurobiologia, University of Pavia, and Istituto di Genetica Molecolare del C.N.R., Piazza botta 10, 27100 Pavia, Italy.
Differentiation. 2008 Jan;76(1):57-65. doi: 10.1111/j.1432-0436.2007.00211.x. Epub 2007 Aug 14.
Perichromatin fibrils represent the morphological expression of transcription and co-transcriptional processing of pre-mRNA. They can be considered, hence, an example of work in progress. High resolution techniques such as electron microscopy demonstrate that perichromatin fibrils play a role as early markers of transcriptional alterations. In this paper, we review some experimental and physiological conditions impairing or modulating transcription as well as their effects on perichromatin fibrils. In all the situations reported, perichromatin fibrils show modifications in their amount and/or their associated proteins. Their movements are also affected, as well as their export or their intra-nuclear storage forms. Perichromatin fibrils therefore represent highly sensitive markers not only for monitoring transcriptional and processing rate but also for identifying the maturation level of pre-mRNA/mRNA occurring in the cell nucleus and the functional correlation with the cellular metabolic state.
染色质外周纤维代表了前体mRNA转录和共转录加工的形态学表现。因此，它们可被视为正在进行的工作的一个例子。诸如电子显微镜等高分辨率技术表明，染色质外周纤维作为转录改变的早期标志物发挥作用。在本文中，我们综述了一些损害或调节转录的实验和生理条件，以及它们对染色质外周纤维的影响。在所有报道的情况下，染色质外周纤维在其数量和/或相关蛋白方面都表现出改变。它们的移动也受到影响，以及它们的输出或核内储存形式。因此，染色质外周纤维不仅是监测转录和加工速率的高度敏感标志物，也是识别细胞核中发生的前体mRNA/ mRNA成熟水平以及与细胞代谢状态功能相关性的高度敏感标志物。
