Prévost Nicolas, Kato Hisashi, Bodin Laurent, Shattil Sanford J
Division of Hematology-Oncology, Department of Medicine, University of California, San Diego, La Jolla, California, USA.
Methods Enzymol. 2007;426:103-15. doi: 10.1016/S0076-6879(07)26006-9.
Integrin-mediated cellular events affect all cell types and functions, in physiological as well as pathological settings. Blood platelets, because of their unique nature, have proven to be a powerful cell model with which to study the adhesive and signaling properties of integrins. The characterization of the structural and molecular mechanisms regulating the main platelet integrin, alphaIIbbeta3, has provided some essential clues as to how integrins are regulated in general. The present chapter details the various protocols and reagents currently in use in our laboratory to study alphaIIbbeta3 adhesive responses and signaling in both human and murine cell models.
整合素介导的细胞事件在生理和病理环境中影响所有细胞类型和功能。血小板因其独特的性质，已被证明是研究整合素黏附与信号特性的强大细胞模型。对调节主要血小板整合素αIIbβ3的结构和分子机制的表征，为整合素的一般调节方式提供了一些重要线索。本章详细介绍了我们实验室目前用于研究人源和鼠源细胞模型中αIIbβ3黏附反应和信号传导的各种实验方案和试剂。
