Klein Eric A, Yung Yuval, Castagnino Paola, Kothapalli Devashish, Assoian Richard K
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
Methods Enzymol. 2007;426:155-75. doi: 10.1016/S0076-6879(07)26008-2.
Cooperative signaling between growth factor receptor tyrosine kinases, integrins, and the actin cytoskeleton is required for activation of the G1-phase cyclin-dependent kinases and progression through G1-phase. Increasing evidence suggests that there is cell type specificity in these cooperative interactions and that the compliance of the underlying substratum can strongly affect adhesion-dependent signaling to the cell cycle. This chapter reviews our current methods for studying how cell type specificity and changes in substratum compliance can contribute to G1-phase cell cycle control. We also describe several of our current analytical procedures.
生长因子受体酪氨酸激酶、整合素和肌动蛋白细胞骨架之间的协同信号传导是激活G1期细胞周期蛋白依赖性激酶并推动细胞通过G1期所必需的。越来越多的证据表明，这些协同相互作用存在细胞类型特异性，并且潜在基质的顺应性可强烈影响对细胞周期的黏附依赖性信号传导。本章回顾了我们目前用于研究细胞类型特异性和基质顺应性变化如何有助于G1期细胞周期调控的方法。我们还描述了一些我们目前的分析程序。
