Zhang Gen-Hua, Zhang Hai-Yun, Deng Shao-Ping, Qin Yu-Mei
Laboratory of Sensory Science, Department of Biological Science, Changshu Institute of Technology, Nansanhuan Road, Changshu City, Jiangsu Province 215500, China.
Acta Histochem. 2007;109(6):486-90. doi: 10.1016/j.acthis.2007.05.003. Epub 2007 Aug 14.
We used alpha-gustducin, a type II taste-cell-specific G protein, to investigate the onset of taste transduction and its relation to the development of the soft palate (SP) and fungiform (FF) papillae taste buds in the mouse. Paraffin wax embedded sections were prepared from the SP and anterior region of the tongue of the mouse from birth until postnatal day (PD) 63. No alpha-gustducin-immunoreactive cells were observed on the day of birth. One day later, alpha-gustducin was immunolocalised in taste buds with pores with a relatively higher frequency recorded in the SP as compared with the FF papillae. The immunoreactive cells were spindle shaped with elongated processes extending from the base to the pore of the taste buds. On PD 7, the number of taste buds containing alpha-gustducin-immunoreactive cells in the SP was three times greater than that of FF papillae. Our results indicate that taste transduction is essentially acquired from the time of birth. Moreover, the onset of taste transduction by the SP taste buds developed earlier than that achieved by taste buds in the FF papillae.
我们使用α - 味导素（一种II型味觉细胞特异性G蛋白）来研究味觉转导的起始及其与小鼠软腭（SP）和菌状乳头（FF）味蕾发育的关系。从出生到出生后第63天，制备小鼠SP和舌前部的石蜡包埋切片。出生当天未观察到α - 味导素免疫反应性细胞。一天后，α - 味导素免疫定位在有味孔的味蕾中，与FF乳头相比，在SP中记录到相对较高的频率。免疫反应性细胞呈纺锤形，有从基部延伸至味蕾孔的细长突起。在出生后第7天，SP中含有α - 味导素免疫反应性细胞的味蕾数量比FF乳头中的多两倍。我们的结果表明，味觉转导基本上从出生时就已获得。此外，SP味蕾的味觉转导起始比FF乳头味蕾更早。
