Zhang Gen-Hua, Zhang Hai-Yun, Deng Shao-Ping, Qin Yu-Mei
Sensory Science Laboratory, Department of Biological Science, Changshu Institute of Technology, 99 Nansanhuan Road, Changshu 215500, Jiangsu Province, People's Republic of China.
Chem Senses. 2008 Apr;33(4):357-62. doi: 10.1093/chemse/bjm093. Epub 2008 Feb 21.
The regional differences between distribution patterns and alpha-gustducin expression patterns of the fungiform (FF) taste buds were investigated in the adult mouse, using hematoxylin-eosin staining and immunofluorescence histochemistry on the most anterior region of the tongue (the first millimeter) through the intermediate region of the tongue (the last 1-4 mm). Paraffin sections were prepared from the tip to posterior regions (anterior and intermediate region containing the FF taste buds) of the adult mouse tongue. Results indicate that there were significant regional differences in size and density of taste buds, the cell counts of the single taste bud, and the alpha-gustducin-immunoreactive taste buds between the 2 regions. The taste bud had a characteristic onion-like appearance, and the alpha-gustducin-immunoreactive cell was spindle shaped with elongated processes extending from the base to the pore of the taste buds. These results provide a detailed insight to better understand regional descriptions of mouse taste bud density and size and alpha-gustducin expression with the mouse model.
利用苏木精-伊红染色和免疫荧光组织化学方法，对成年小鼠舌最前端区域（前1毫米）至舌中间区域（后1 - 4毫米）的菌状（FF）味蕾分布模式和α-味导素表达模式的区域差异进行了研究。从成年小鼠舌的尖端到后部区域（包含FF味蕾的前部和中间区域）制备石蜡切片。结果表明，两个区域之间在味蕾的大小和密度、单个味蕾的细胞计数以及α-味导素免疫反应性味蕾方面存在显著的区域差异。味蕾呈典型的洋葱样外观，α-味导素免疫反应性细胞呈纺锤形，有从基部延伸至味蕾孔的细长突起。这些结果为利用小鼠模型更好地理解小鼠味蕾密度和大小的区域描述以及α-味导素表达提供了详细的见解。
