肥胖受试者网膜肿瘤坏死因子-α蛋白与血浆纤溶酶原激活物抑制因子-1之间的相关性。

Correlation between omental TNF-alpha protein and plasma PAI-1 in obesity subjects.

作者信息

Cao Yan Li, Wang Yu Xia, Wang Di Fei, Meng Xin, Zhang Jin

机构信息

Department of Endocrinology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.

出版信息

Int J Cardiol. 2008 Aug 29;128(3):399-405. doi: 10.1016/j.ijcard.2007.05.033. Epub 2007 Aug 15.

DOI:10.1016/j.ijcard.2007.05.033

PMID:17698217

Abstract

BACKGROUND

The role of TNF-alpha in contributing to obesity-associated cardiovascular and metabolic risk has gained much attention.

MATERIALS AND METHODS

Paired biopsies of omental and subcutaneous fat were collected from 16 lean subjects and 32 central obesity subjects. The expression of TNF-alpha in omental and subcutaneous fat was quantified by western blotting method, and correlations with plasma PAI-1, homeostasis model assessment insulin resistance (HOMA-IR), and lipid were investigated.

RESULTS

In obese female, TNF-alpha expression was higher in the omental than in the subcutaneous fat tissue. There was no significant difference in the levels of TNF-alpha between subcutaneous and visceral fat in obese male. Significant positive correlations were found between omental TNF-alpha protein and plasma PAI-1 levels in obesity. In obese female subjects, omental TNF-alpha protein levels showed a close association with most of the parameters studied: fasting glucose (r=0.541, P<0.05); fasting insulin (r=0.599, P<0.01); HOMA-IR (r=0.546, P<0.05); triglycerides (r=0.469, P<0.05); HDL-cholesterol (r=-0.759, P<0.01). In obese male population, correlations between omental TNF-alpha protein levels and fasting glucose (r=0.762, P<0.01); fasting insulin (r=0.622, P<0.05); triglycerides (r=0.650, P<0.05); HDL-cholesterol (r=-0.880, P<0.01) were found.

CONCLUSION

Omental TNF-alpha may play a key role in contributing to cardiovascular risk in central obesity subjects.

摘要

背景

肿瘤坏死因子-α（TNF-α）在肥胖相关心血管和代谢风险中的作用已备受关注。

材料与方法

从16名瘦素正常受试者和32名中心性肥胖受试者中采集网膜脂肪和皮下脂肪的配对活检样本。采用蛋白质印迹法对网膜脂肪和皮下脂肪中TNF-α的表达进行定量，并研究其与血浆纤溶酶原激活物抑制因子-1（PAI-1）、稳态模型评估胰岛素抵抗（HOMA-IR）及血脂的相关性。

结果

在肥胖女性中，网膜脂肪组织中TNF-α的表达高于皮下脂肪组织。肥胖男性皮下脂肪和内脏脂肪中TNF-α水平无显著差异。肥胖者网膜TNF-α蛋白与血浆PAI-1水平之间存在显著正相关。在肥胖女性受试者中，网膜TNF-α蛋白水平与大多数研究参数密切相关：空腹血糖（r = 0.541，P < 0.05）；空腹胰岛素（r = 0.599，P < 0.01）；HOMA-IR（r = 0.546，P < 0.05）；甘油三酯（r = 0.469，P < 0.05）；高密度脂蛋白胆固醇（r = -0.759，P < 0.01）。在肥胖男性群体中，发现网膜TNF-α蛋白水平与空腹血糖（r = 0.762，P < 0.01）；空腹胰岛素（r = 0.622，P < 0.05）；甘油三酯（r = 0.650，P < 0.05）；高密度脂蛋白胆固醇（r = -0.880，P < 0.01）之间存在相关性。