Ochi Masaaki, Osawa Haruhiko, Hirota Yushi, Hara Kazuo, Tabara Yasuharu, Tokuyama Yoshiharu, Shimizu Ikki, Kanatsuka Azuma, Fujii Yasuhisa, Ohashi Jun, Miki Tetsuro, Nakamura Naoto, Kadowaki Takashi, Itakura Mitsuo, Kasuga Masato, Makino Hideichi
Department of Molecular and Genetic Medicine, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
Diabetes. 2007 Nov;56(11):2834-8. doi: 10.2337/db06-1157. Epub 2007 Aug 13.
Resistin is an adipocyte-secreted cytokine associated with insulin resistance in mice. We previously reported that the G/G genotype of a resistin single nucleotide polymorphism (SNP) at -420 increases type 2 diabetes susceptibility by enhancing its promoter activity. The aim of the present study was to determine the relevance of SNP -120 in a large number of subjects.
We examined 2,610 type 2 diabetic case and 2,502 control subjects. The relation between SNP -420 and the age of type 2 diabetes onset was further analyzed by adding 237 type 2 diabetic subjects with age of onset <or=40 years.
When analyzed without considering subject age, the SNP -420 genotype was not associated with type 2 diabetes. Since we reported that the onset of type 2 diabetes was earlier in G/G genotype, we analyzed the data using a trend test for age intervals of 10 years. The frequency of G/G genotype differed among age grades in type 2 diabetes (P = 0.037) and appeared to be higher in younger grades. In type 2 diabetes, G/G genotype was more frequent in subjects aged <40 years than in those aged >or=40 years (G/G vs. C/C, P = 0.003). In a total of 2,430 type 2 diabetic subjects with age of onset <60 years, the trend test showed that the G/G genotype had an increasing linear trend as the age grade of type 2 diabetes onset became younger (P = 0.0379). In control subjects, the frequency of C/G genotype showed an increasing linear trend with increasing age (P = 0.010).
The G/G genotype frequency of resistin SNP -420 appears to be increased in younger-onset type 2 diabetic subjects.
抵抗素是一种由脂肪细胞分泌的细胞因子，与小鼠的胰岛素抵抗相关。我们之前报道，抵抗素单核苷酸多态性（SNP）-420位点的G/G基因型通过增强其启动子活性增加2型糖尿病易感性。本研究的目的是在大量受试者中确定SNP -120的相关性。
我们检测了2610例2型糖尿病患者和2502例对照受试者。通过纳入237例发病年龄≤40岁的2型糖尿病患者，进一步分析SNP -420与2型糖尿病发病年龄之间的关系。
在不考虑受试者年龄的情况下进行分析时，SNP -420基因型与2型糖尿病无关。由于我们之前报道2型糖尿病发病在G/G基因型中更早，我们使用10年年龄区间的趋势检验分析数据。2型糖尿病患者中G/G基因型频率在不同年龄组间存在差异（P = 0.037），且在较年轻组中似乎更高。在2型糖尿病患者中，年龄<40岁的受试者中G/G基因型比年龄≥40岁的受试者更常见（G/G与C/C相比，P = 0.003）。在总共2430例发病年龄<60岁的2型糖尿病患者中，趋势检验显示随着2型糖尿病发病年龄组变年轻，G/G基因型呈上升线性趋势（P = 0.0379）。在对照受试者中，C/G基因型频率随年龄增加呈上升线性趋势（P = 0.010）。
抵抗素SNP -420的G/G基因型频率在发病年龄较轻的2型糖尿病患者中似乎增加。
