Takhshid Mohammad Ali, Zare Zinab
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
J Diabetes Metab Disord. 2015 Apr 28;14:37. doi: 10.1186/s40200-015-0165-y. eCollection 2015.
Resistin is a circulating adipokine with insulin-antagonizing effects. The aim of this study was to investigate the relationship between the single nucleotide polymorphism (SNP) -420C > G in the resistin gene with serum resistin levels, insulin resistance, and risk of gestational diabetes (GDM) in Iranian population.
75 GDM patients and 70 healthy pregnant women were enrolled in this study. Genotyping for SNP- 420C > G in the resistin gene was performed by the polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. Serum resistin and insulin were measured by immunoassay. Blood glucose levels and lipid profile were measured by enzymatic methods. Homeostasis model of assessment for insulin resistance (HOMA-IR) were calculated.
GG genotype and G allele of SNP-420C > G were more frequent in GDM patients compared to non-GDM subjects. Serum resistin level was similar in GDM and non-GDM patients. The serum levels of resistin in GDM and non-GDM women with GG genotype were similar to those with GC + CC genotype. Multivariate logistic regression analysis after adjusting for confounding factors showed a higher susceptibility to GDM in patients with GG genotype compared to subjects with GG + GT genotype (odds ratio = 4.59, 95% CI; 1.96-10.71, p = 0.00). Serum resistin level was correlated with serum triglyceride, total and low density lipoprotein (LDL) cholesterol (p < 0.05) in GDM patients. No significant association was found between serum resistin, insulin resistance, and SNP-420C > G.
The SNP-420C/G of resistin gene is associated with genetic susceptibility to GDM in our population. Further studies are necessary to confirm the role of this polymorphism in pathogenesis of GDM and to explore potential mechanisms by which it modulates susceptibility to GDM.
抵抗素是一种具有胰岛素拮抗作用的循环脂肪因子。本研究旨在探讨伊朗人群中抵抗素基因单核苷酸多态性（SNP）-420C＞G与血清抵抗素水平、胰岛素抵抗及妊娠期糖尿病（GDM）风险之间的关系。
本研究纳入了75例GDM患者和70例健康孕妇。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对抵抗素基因SNP-420C＞G进行基因分型。采用免疫分析法测定血清抵抗素和胰岛素水平。采用酶法测定血糖水平和血脂谱。计算胰岛素抵抗的稳态模型评估（HOMA-IR）。
与非GDM受试者相比，GDM患者中SNP-420C＞G的GG基因型和G等位基因更为常见。GDM患者和非GDM患者的血清抵抗素水平相似。GG基因型的GDM和非GDM女性的血清抵抗素水平与GC+CC基因型的女性相似。在调整混杂因素后的多因素逻辑回归分析显示，与GG+GT基因型的受试者相比，GG基因型的患者对GDM的易感性更高（优势比=4.59，95%可信区间；1.96-10.71，p=0.00）。GDM患者的血清抵抗素水平与血清甘油三酯、总胆固醇和低密度脂蛋白（LDL）胆固醇相关（p＜0.05）。血清抵抗素、胰岛素抵抗与SNP-420C＞G之间未发现显著关联。
抵抗素基因的SNP-420C/G与我们人群中GDM的遗传易感性相关。需要进一步研究以证实该多态性在GDM发病机制中的作用，并探索其调节GDM易感性的潜在机制。
