Hosseini A, Hogg D A
Department of Anatomy, University of Glasgow, Scotland, UK.
J Anat. 1991 Aug;177:169-78.
In order to study the effects of paralysis on the development of bone, chick embryos were paralysed at 6 days of incubation and the pattern of histogenesis of the tibia was compared with that of control embryos by histology, scanning electron microscopy and morphometry. Up to 11 days of incubation the histological features of chondrification, initial perichondrial ossification and invasion by the vascular bud showed no differences. After this time the paralysed embryos exhibited a reduction in the formation of bone tissue and a reduced development of the fibular crest. The spread of cartilage resorption was also markedly reduced. In addition, scanning electron microscopy suggested a reduction in resorption of the innermost layers of bone. Blood vessels in the marrow cavity appeared smaller and those within developing osteones appeared larger than in the controls. In the paralysed embryos, morphometry confirmed a significant reduction in total volume of the tibia together with changes in its volumetric composition resulting from reduction in bone formation, cartilage formation and cartilage resorption.
为了研究麻痹对骨骼发育的影响，在孵化第6天时使鸡胚麻痹，并通过组织学、扫描电子显微镜和形态计量学将胫骨的组织发生模式与对照胚胎进行比较。直至孵化第11天，软骨化、初始软骨膜骨化和血管芽侵入的组织学特征均无差异。在此之后，麻痹的胚胎表现出骨组织形成减少以及腓骨嵴发育减弱。软骨吸收的范围也明显减小。此外，扫描电子显微镜显示骨最内层的吸收减少。骨髓腔内的血管看起来比对照胚胎中的小，而发育中的骨单位内的血管看起来比对照胚胎中的大。在麻痹的胚胎中，形态计量学证实胫骨总体积显著减小，同时由于骨形成、软骨形成和软骨吸收减少，其体积组成发生了变化。
