Güney Mehmet, Demirin Hilmi, Oral Baha, Ozgüner Meltem, Bayhan Gökhan, Altuntas Irfan
Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
Hum Exp Toxicol. 2007 Jun;26(6):491-8. doi: 10.1177/0960327106077505.
We have investigated the effect of subchronic administration of methidathion (MD) on ovary evaluated ameliorating effects of vitamins E and C against MD toxicity. Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD + Vit group). MD and MD + Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P < 0.005). Serum MDA decreased significantly in the MD + Vit group compared with the MD group (P < 0.05). The activities of ChE decreased significantly both in the MD and MD + Vit groups compared with the controls ( P < 0.05). However, the decrease in the MD + Vit groups was less than in the MD group; the ChE activity in the MD + Vit group was significantly higher compared with MD group (P < 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P < 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P < 0.05). Follicle counts in MD + Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P < 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage.
我们研究了亚慢性给予杀扑磷（MD）对卵巢的影响，并评估了维生素E和维生素C对MD毒性的改善作用。实验组如下：对照组；一组接受5mg/kg体重MD治疗（MD组）；以及一组接受5mg/kg体重MD加维生素E和维生素C治疗（MD + Vit组）。MD组和MD + Vit组每周5天通过灌胃给予MD，持续4周，剂量水平为5mg/kg/天，使用玉米油作为载体。研究了血清丙二醛（MDA：脂质过氧化指标）浓度、血清胆碱酯酶（ChE）活性和卵巢组织病理学。与对照组相比，MD组的MDA水平显著升高（P < 0.005）。与MD组相比，MD + Vit组的血清MDA显著降低（P < 0.05）。与对照组相比，MD组和MD + Vit组的ChE活性均显著降低（P < 0.05）。然而，MD + Vit组的降低幅度小于MD组；MD + Vit组的ChE活性与MD组相比显著更高（P < 0.05）。与对照组相比，MD组的卵巢卵泡数量显著更低（P < 0.05）。MD组的闭锁卵泡数量显著高于对照组（P < 0.05）。MD + Vit组的卵泡计数显示，与MD组相比，所有类型的卵巢卵泡均显著更高，且闭锁卵泡数量显著减少（P < 0.05）。总之，亚慢性给予MD会导致卵巢损伤，此外，脂质过氧化可能是MD诱导毒性所涉及的分子机制之一。MD给药后用维生素E和维生素C治疗可降低脂质过氧化和卵巢损伤。
