Azadniv Mitra, Dugger Kari, Bowers William J, Weaver Casey, Crispe Ian N
David H Smith Center for Microbiology and Immunology, Aab Institute for Biomedical Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA.
Int Immunol. 2007 Oct;19(10):1165-73. doi: 10.1093/intimm/dxm086. Epub 2007 Aug 14.
After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and revealed several unexpected features. First, the T cells localized to the site of vector injection, as well as the draining lymph node within 24-48 h. Second, the major site to which T cells later redistributed were intra-abdominal lymphoid organs, including milky spots, mesenteric and lumbar lymph nodes. We determined the relationship between bioluminescent signal and antigen-specific T cell numbers in various lymphoid organs, and concluded that bioluminescent signal is a valid surrogate measure of T cell abundance in superficial lymph nodes, but not in deeper structures such as the spleen.
激活后，抗原特异性T细胞群体在抗原表达部位、局部淋巴结构以及其他淋巴和非淋巴器官之间流动。在本研究中，我们记录了使用单纯疱疹病毒扩增载体递送抗原后CD8(+) T细胞反应的体内动态，并揭示了几个意外特征。首先，T细胞在24 - 48小时内定位于载体注射部位以及引流淋巴结。其次，T细胞后来重新分布的主要部位是腹腔内淋巴器官，包括乳斑、肠系膜和腰淋巴结。我们确定了各种淋巴器官中生物发光信号与抗原特异性T细胞数量之间的关系，并得出结论：生物发光信号是浅表淋巴结中T细胞丰度的有效替代指标，但不适用于脾脏等更深层结构。
