Yamaguchi T, Kajikawa T, Kimoto E
Department of Chemistry, Faculty of Science, Fukuoka University.
J Biochem. 1991 Sep;110(3):355-9. doi: 10.1093/oxfordjournals.jbchem.a123585.
When human erythrocytes were subjected to hydrostatic pressure (1.1-2.0 kbar), it was found that membrane vesicles were released from the red cells above 1.4 kbar. As with hemolysis under high pressure, the amount of released vesicles was increased with increasing pressure but decreased by the cross-linking of membrane proteins with diamide. Vesicles obtained at 2.0 kbar were heterogeneous in size but similar to intact erythrocytes in phospholipid composition. Although it has been reported that spectrin-free vesicles are released by echinocytogenic agents, pressure-induced vesicles did contain considerable and similar amounts of spectrin irrespective of the difference in size. These results suggest that vesiculation by high pressure is associated with the disruption of the membrane skeleton, as previously seen in pressure-induced hemolysis [Yamaguchi et al. (1989) J. Biochem. 106, 1080-1085].
当人红细胞受到静水压力（1.1 - 2.0千巴）作用时，发现压力高于1.4千巴时红细胞会释放出膜囊泡。与高压下的溶血情况一样，释放出的囊泡数量随压力增加而增多，但膜蛋白经二酰胺交联后数量会减少。在2.0千巴压力下获得的囊泡大小不一，但磷脂组成与完整红细胞相似。尽管有报道称棘状红细胞生成剂可释放无血影蛋白的囊泡，但压力诱导产生的囊泡无论大小差异，都含有相当数量且相似量的血影蛋白。这些结果表明，高压诱导的囊泡形成与膜骨架的破坏有关，正如之前在压力诱导的溶血过程中所观察到的那样[山口等人（1989年）《生物化学杂志》106卷，第1080 - 1085页]。
