Clinical significance of urinary laminin P1 in diabetic patients.

The urinary concentrations of laminin fragment P1 (L-P1), a major component of laminin, were determined in diabetic patients without diabetic nephropathy and healthy controls. In the control subjects, urinary L-P1 increased with age, especially over 60 years of age. A significant increase of urinary L-P1 was observed in diabetics aged less than 50 years. Neither urinary albumin nor N-acetyl-beta-D-glucosaminidase correlated to the urinary L-P1 level. We used immunohistochemistry to locate L-P1 in the cortex of human kidneys. In non-diabetic kidneys, the glomerular and tubular basement membranes, mesangium, and Bowman's capsule were stained. In the diabetic kidney, more was stained, including the mesangial expansion and the thickened capillary basement membranes.