Park M-J, Choi H-B, Jang J-P, Kim H-J, Kim Y-J, Eom K-S, Lee S, Kim D-W, Lee J-W, Min W-S, Kim C-C, Kim T-G
Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Bone Marrow Transplant. 2007 Oct;40(8):723-8. doi: 10.1038/sj.bmt.1705808. Epub 2007 Aug 13.
Minor histocompatibility antigens (mHags) are polymorphic peptides presented to T lymphocytes restricted by the MHC molecule. It has been reported that disparities of mHags are a potential risk factor for GVHD after hematopoietic SCT (HSCT). Here we observed allelic frequencies of HA-1, -2 and -8 in 139 Korean healthy individuals using PCR-sequence-specific primers, and analyzed the correlation between disparity of these mHags and acute GVHD (aGVHD) in 54 patients who underwent HSCT from unrelated HLA-identical donors. The allelic frequencies in Korean healthy individuals were 39.6 and 60.4% for HA-1(H) and HA-1(R), 92.4 and 7.6% for HA-2(M) and HA-2(V), 36.7 and 63.3% for HA-8(R) and HA-8(P), respectively. The frequencies of mHags incompatibility known to be associated with aGVHD were 16.7% in HA-1, 0% in HA-2 and 25.9% in HA-8. However, the statistically significant association of aGVHD with these mHags incompatibility was not found between healthy donors and leukemia patients after unrelated HSCT. This first report about mHags in Koreans may be helpful in further defining the clinical impact of mHags disparities in HSCT and in comparing with other populations.
次要组织相容性抗原（mHags）是由主要组织相容性复合体（MHC）分子呈递给T淋巴细胞的多态性肽段。据报道，mHags的差异是造血干细胞移植（HSCT）后发生移植物抗宿主病（GVHD）的潜在危险因素。在此，我们使用PCR序列特异性引物观察了139名韩国健康个体中HA-1、-2和-8的等位基因频率，并分析了这些mHags差异与54例接受无关HLA匹配供体HSCT患者的急性GVHD（aGVHD）之间的相关性。韩国健康个体中，HA-1（H）和HA-1（R）的等位基因频率分别为39.6%和60.4%，HA-2（M）和HA-2（V）的分别为92.4%和7.6%，HA-8（R）和HA-8（P）的分别为36.7%和63.3%。已知与aGVHD相关的mHags不相容频率在HA-1中为16.7%，在HA-2中为0%，在HA-8中为25.9%。然而，在无关HSCT后的健康供体和白血病患者之间，未发现aGVHD与这些mHags不相容之间存在统计学显著关联。这份关于韩国人mHags的首次报告可能有助于进一步明确mHags差异在HSCT中的临床影响，并与其他人群进行比较。
