Príncipe Analía, Alvarez Florencia, Castro Marina G, Zacchi Lucı F, Fischer Sonia E, Mori Gladys B, Jofré Edgardo
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-Km 601-5800, Río Cuarto-Córdoba, Argentina.
Curr Microbiol. 2007 Oct;55(4):314-22. doi: 10.1007/s00284-006-0654-9. Epub 2007 Aug 13.
A bacterial collection of approximately one thousand native strains, isolated from saline soils of Cordoba province (Argentina), was established. From this collection, a screening to identify those strains showing plant growth promotion and biocontrol activities, as well as salt tolerance, was performed. Eight native strains tolerant to 1 M: NaCl and displaying plant growth promotion and/or biocontrol features were selected for further characterization. Strains MEP(2 )18, MRP(2 )26, MEP(2 )11a, MEP(3 )1, and MEP(3 )3b significantly increased the growth of maize seedlings under normal and saline conditions, whereas isolates ARP(2 )3, AEP(1 )5, and ARP(2 )6 were able to increase the root dry weight of agropyre under saline conditions. On the other hand, strains MEP(2 )18 and ARP(2 )3 showed antagonistic activity against phytopathogenic fungi belonging to Sclerotinia and Fusarium genus. Antifungal activity was found in cell-free supernatants, and it was heat and protease resistant. Strains MEP(2)18 and ARP(2)3 were identified as Bacillus sp. and strains MEP(2)11a and MEP(3)3b as Ochrobactrum sp. according to the sequence analysis of 16S rRNA gene.
建立了一个从科尔多瓦省（阿根廷）盐渍土中分离出的约1000株本地菌株的细菌库。从这个库中进行筛选，以鉴定那些具有促进植物生长、生物防治活性以及耐盐性的菌株。选择了8株耐1M NaCl并具有促进植物生长和/或生物防治特性的本地菌株进行进一步表征。菌株MEP(2)18、MRP(2)26、MEP(2)11a、MEP(3)1和MEP(3)3b在正常和盐渍条件下显著促进了玉米幼苗的生长，而分离株ARP(2)3、AEP(1)5和ARP(2)6在盐渍条件下能够增加冰草的根干重。另一方面，菌株MEP(2)18和ARP(2)3对核盘菌属和镰刀菌属的植物病原真菌表现出拮抗活性。在无细胞上清液中发现了抗真菌活性，并且该活性对热和蛋白酶具有抗性。根据16S rRNA基因的序列分析，菌株MEP(2)18和ARP(2)3被鉴定为芽孢杆菌属，菌株MEP(2)11a和MEP(3)3b被鉴定为苍白杆菌属。
