中国脑膜炎奈瑟菌A、B和C群以外血清群的基因分型分析

Genotypic analysis of serogroups other than A, B or C of Neisseria meningitidis in China.

作者信息

Yang Jie, Zhang Xiaobing, Xu Xingye, Xu Li, Li Machao, Yang Li, Zhu Yafang, Shao Zhujun, Liang Xiaofeng, Xu Jianguo, Wang Yu, Jin Qi

机构信息

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.

出版信息

Scand J Infect Dis. 2007;39(9):819-21. doi: 10.1080/00365540701299616.

DOI:10.1080/00365540701299616

PMID:17701722

Abstract

To serologically and genetically characterize other serogroups (except A, B, and C) of Neisseria meningitidis isolates in China, we collected 56 strains of other serogroups, identified by serogroup typing and multilocus sequence typing (MLST). All of them are non-invasive isolates. The serogroups of the 56 Chinese isolates were W135 (11 isolates), Y (4), X (15), 29E (15), D (1), H (4), I (3), K (2), and non-groupable (1). By MLST, 34 different sequence types (STs) were identified, 28 of which were not found in the MLST database as of July 2006 and seemed to be unique to China. Statistical analysis of the MLST results revealed that, although the Chinese isolates seemed to be genetically divergent, they could be classified into 5 major clonal groups and other minor groups. Among these isolates, none of the well-documented ST complexes found worldwide was present.

摘要

为了从血清学和遗传学角度对中国分离出的脑膜炎奈瑟菌其他血清群（A、B 和 C 群除外）进行特征分析，我们收集了 56 株其他血清群菌株，通过血清群分型和多位点序列分型（MLST）进行鉴定。所有菌株均为非侵袭性分离株。56 株中国分离株的血清群分别为 W135（11 株）、Y（4 株）、X（15 株）、29E（15 株）、D（1 株）、H（4 株）、I（3 株）、K（2 株）和不可分组（1 株）。通过 MLST，鉴定出 34 种不同的序列型（STs），其中 28 种截至 2006 年 7 月在 MLST 数据库中未被发现，似乎是中国特有的。对 MLST 结果的统计分析表明，尽管中国分离株在基因上似乎存在差异，但它们可分为 5 个主要克隆群和其他次要群。在这些分离株中，未发现全球范围内记录的任何 ST 复合体。