Bäckman P, Wadsö I
Thermochemistry Division, University of Lund, Sweden.
J Biochem Biophys Methods. 1991 Dec;23(4):283-93. doi: 10.1016/0165-022x(91)90003-f.
A method is described for simultaneous measurements of heat production rate, oxygen activity and pH, using a 3-ml stirred microcalorimetric titration/perfusion vessel fitted with a polarographic oxygen sensor and a combination pH-electrode. Heat production rate (+/- 0.3 microW), pH (+/- 0.01) and oxygen concentration (+/- 4 mumol/l) could be measured without any cross interference between the three signals. Results from measurements on suspensions of T-lymphoma cells and E. coli are presented.
