用于通过毛细管电泳分离双链DNA片段的新型羟乙基纤维素接枝聚丙烯酰胺共聚物
Novel hydroxyethylcellulose-graft-poly acrylamide copolymer for separation of double-stranded DNA fragments by CE.
作者信息
Yang Runmiao, Wang Yanmei, Zhou Dan
机构信息
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
出版信息
Electrophoresis. 2007 Sep;28(18):3223-31. doi: 10.1002/elps.200600818.
A novel separation medium, hydroxyethylcellulose-graft-polyacrylamide (HEC-g-PAM) synthesized by atom transfer radical polymerization (ATRP), used for dsDNA separation by CE is presented. The separation performance of HEC-g-PAM, which has the same graft density and different graft length, has been investigated in Tris-boric acid-EDTA (TBE) buffer solvent mixtures. The temperature-dependent rheological behavior of HEC-g-PAM was also studied by steady-shear rheometry. The results showed that dsDNA fragments between 72 and 1353 bp was achieved with a 30 cm effective capillary length at 150 V/cm using this type of graft copolymer as a separation medium in bare fused-silica capillaries, and separation improvement is obtained in HEC-g-PAM compared with HEC and poly(dimethylacrylamide (PDMA).
介绍了一种通过原子转移自由基聚合（ATRP）合成的新型分离介质，即羟乙基纤维素接枝聚丙烯酰胺（HEC-g-PAM），用于毛细管电泳分离双链DNA（dsDNA）。在 Tris-硼酸-EDTA（TBE）缓冲溶剂混合物中，研究了具有相同接枝密度和不同接枝长度的 HEC-g-PAM 的分离性能。还通过稳态剪切流变仪研究了 HEC-g-PAM 的温度依赖性流变行为。结果表明，在裸熔融石英毛细管中使用这种接枝共聚物作为分离介质，在 150 V/cm 的电压下，有效毛细管长度为 30 cm 时，可实现 72 至 1353 bp 的双链 DNA 片段分离，与 HEC 和聚二甲基丙烯酰胺（PDMA）相比，HEC-g-PAM 的分离效果有所改善。
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