羟乙基纤维素接枝聚（N，N - 二甲基丙烯酰胺）共聚物作为毛细管电泳的多功能分离介质。

Hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer as a multifunctional separation medium for CE.

作者信息

Peng Shuhua, Shi Ronghua, Yang Runmiao, Zhou Dan, Wang Yanmei

机构信息

Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China, Hefei, PR China.

出版信息

Electrophoresis. 2008 Nov;29(21):4351-4. doi: 10.1002/elps.200800149.

DOI:10.1002/elps.200800149

PMID:18942678

Abstract

A new multifunctional separation medium, hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer synthesized in our laboratory for application in both basic protein separation and dsDNA separation by CE, is presented in this paper. As a noncovalent coating, this medium showed a powerful capability in resisting basic protein adsorption. Highly efficient and rapid protein separation had been obtained at four different pH values. Meanwhile, the 11 fragments of the dsDNA sample could be baseline separated using this grafted copolymer as sieving matrix at an appropriate concentration.

摘要

本文介绍了一种新型多功能分离介质，即我们实验室合成的羟乙基纤维素接枝聚（N，N - 二甲基丙烯酰胺）共聚物，用于毛细管电泳中碱性蛋白质分离和双链DNA分离。作为一种非共价涂层，该介质在抵抗碱性蛋白质吸附方面表现出强大的能力。在四个不同的pH值下都实现了高效快速的蛋白质分离。同时，以该接枝共聚物为筛分基质，在适当浓度下，双链DNA样品的11个片段能够实现基线分离。