Wang Y, Dai S-D, Qi F-J, Xu H-T, Wang E-H
Department of Pathology, College of Basic Medical Sciences, China Medical University and Department of Pathology, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
J Clin Pathol. 2008 Feb;61(2):192-6. doi: 10.1136/jcp.2007.050401. Epub 2007 Aug 17.
To investigate the significance of p53 protein expression and genetic mutations in two primary cell types in pulmonary sclerosing haemangioma (PSH).
p53 protein expression in polygonal cells and cuboidal cells in 19 patients with PSH was detected using immunohistochemistry. The two major cell types were captured using laser capture microdissection technology. Mutations in the p53 gene (exons 5-8) were examined using single-stranded conformation polymorphism and DNA sequencing analysis.
p53 protein expression and gene mutations were observed in 15.8% (3/19) of cases. In these cases, p53 protein was expressed in the nucleus of both cell types, with higher expression levels and mutation rates in polygonal cells than in surface cuboidal cells. Two cases showed mutation only in the polygonal cells, while one case showed double (separate) mutations in both the polygonal and cuboidal cells.
p53 mutation was exhibited in PSH. The mutation rate in polygonal cells was higher than that in surface cuboidal cells.
探讨p53蛋白表达及基因突变在肺硬化性血管瘤（PSH）两种主要细胞类型中的意义。
采用免疫组织化学法检测19例PSH患者中多边形细胞和立方形细胞的p53蛋白表达。运用激光捕获显微切割技术获取这两种主要细胞类型。采用单链构象多态性和DNA测序分析检测p53基因（第5-8外显子）的突变情况。
15.8%（3/19）的病例中观察到p53蛋白表达及基因突变。在这些病例中，两种细胞类型的细胞核均有p53蛋白表达，多边形细胞中的表达水平及突变率高于表面立方形细胞。2例仅在多边形细胞中出现突变，1例在多边形细胞和立方形细胞中均出现双（独立）突变。
PSH中存在p53突变。多边形细胞中的突变率高于表面立方形细胞。
