Jagannath Swaminathan, Sabareesh Varatharajan
Department of Biotechnology, Vellore Institute of Technology, Vellore 632 014, India.
Rapid Commun Mass Spectrom. 2007;21(18):3033-8. doi: 10.1002/rcm.3179.
The software Peptide Fragment Ion Analyser (PFIA) aids in the analysis and interpretation of tandem mass spectrometric data of peptides. The software package has been designed to facilitate the analysis of product ions derived from acyclic and cyclic peptide natural products that possess unusual amino acid residues and are heavily post-translationally modified. The software consists of two programmes: (a) PFIA-I lists the amino acid compositions and their corresponding product ion types for 'a queried m/z value' (z = +1) and (b) PFIA-II displays fragmentation pattern diagram(s) and lists all sequence-specific product ion types for the protonated adduct of 'a queried sequence'. The unique feature of PFIA-II is its ability to handle cyclic peptides. The two programmes used in combination can prove helpful for deriving peak assignments in the de novo sequencing of novel peptides.
肽片段离子分析仪（PFIA）软件有助于肽的串联质谱数据的分析和解读。该软件包旨在便于分析源自无环和环肽天然产物的产物离子，这些天然产物含有不寻常的氨基酸残基且经过大量翻译后修饰。该软件由两个程序组成：（a）PFIA - I列出“查询的m/z值”（z = +1）的氨基酸组成及其相应的产物离子类型，以及（b）PFIA - II显示碎片模式图，并列出“查询序列”的质子化加合物的所有序列特异性产物离子类型。PFIA - II的独特功能是其处理环肽的能力。结合使用这两个程序有助于在新肽的从头测序中进行峰归属。
