Basu Samar, Meisert Isabelle, Eggensperger Elisabeth, Krieger Elisabeth, Krenn Claus G
Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden.
Redox Rep. 2007;12(4):195-202. doi: 10.1179/135100007X200281.
Ischaemia-reperfusion injury resulting from interruption and restoration of blood flow might be related to free radical mediated oxidative stress and inflammation, and subsequently to post-surgery related complications. We studied the impact of renal transplantation on oxidative stress and inflammation by measuring F(2)-isoprostanes and prostaglandin F(2alpha), respectively, during transplantation and post-surgery. Additionally, due to earlier observations, two dissimilar anaesthetic agents (thiopentone and propofol) were compared to determine their antioxidative capacity rather than their anaesthetic properties. Blood samples were collected before, post-intubation, immediately, 30, 60,120, 240 min, and 12 and 24 h after reperfusion. Oxidative stress and inflammatory response were detected by measuring 8-iso-PGF(2alpha) (a major F(2)-isoprostane and a biomarker of oxidative stress) and 15-keto-dihydro-PGF(2alpha) (a major metabolite of PGF(2alpha) and a biomarker of COX-mediated inflammatory response), respectively. Reperfusion of the transplanted graft significantly increased plasma levels of 8-iso-PGF(2alpha). PGF(2alpha) metabolite levels, although elevated, did not reach statistical significance. In addition, significantly lower levels of 8-iso-PGF(2a) were observed in the propofol group compared to the thiopentone group. Together, these findings underline an augmented oxidative stress activity following an inflammatory response after human renal transplantation. Furthermore, propofol a well-known anaesthetic, counteracted oxidative stress by lowering the formation of a major F(2)-isoprostane.
血流中断和恢复所导致的缺血再灌注损伤可能与自由基介导的氧化应激和炎症相关，进而与术后相关并发症有关。我们通过在移植过程中和术后分别测量F(2)-异前列腺素和前列腺素F(2α)，研究了肾移植对氧化应激和炎症的影响。此外，基于早期观察结果，比较了两种不同的麻醉剂（硫喷妥钠和丙泊酚）以确定它们的抗氧化能力而非麻醉特性。在插管前、插管后、再灌注后即刻、30、60、120、240分钟以及12和24小时采集血样。分别通过测量8-异前列腺素F(2α)（一种主要的F(2)-异前列腺素和氧化应激的生物标志物）和15-酮二氢前列腺素F(2α)（前列腺素F(2α)的一种主要代谢产物和COX介导的炎症反应的生物标志物）来检测氧化应激和炎症反应。移植肾的再灌注显著提高了血浆中8-异前列腺素F(2α)的水平。前列腺素F(2α)代谢物水平虽有所升高，但未达到统计学显著性。此外，与硫喷妥钠组相比，丙泊酚组中8-异前列腺素F(2α)的水平显著更低。这些发现共同强调了人类肾移植后炎症反应后氧化应激活性增强。此外，丙泊酚作为一种知名的麻醉剂，通过降低主要F(2)-异前列腺素的形成来对抗氧化应激。
