da Rocha Raquel A, Bricarello Patrizia A, da Rocha Gilberto P, Amarante Alessandro F T
Departamento de Parasitologia, Instituto de Biociências, UNESP, Botucatu, SP, Brazil.
Rev Bras Parasitol Vet. 2007 Apr-Jun;16(2):77-82.
The purpose of the experiment was to evaluate infective Trichostrongylus colubriformis larvae vertical migration in two forage grass species. Experimental modules formed by eight plots, established with Brachiaria decumbens cv. Australian and Panicum maximum cv. Aruana, were used in the study, totaling four plots for each grass species. Each plot was divided into six 30 x 30 cm subplots. Larval migration was evaluated in the four seasons of the year, in different plant strata (0-7, 7-14, 14-21, 21-28 and above 28 cm). Four feces deposits were made, one in each season of the year, in the middle of 30-cm tall forage. The feces were collected from the forage ten days after each feces deposit in the experimental subplots. Grass height was measured in each of the strata immediately before the collections. The forage of the different strata was cut from an area measuring 10-cm in radius. The feces were collected manually from the subplots. There was a grass species and grass stratum interaction in the deposit made in autumn (P<0.05). During that season, most of the larvae were recovered from the Brachiaria grass base; meanwhile, at the forage apex, the biggest average was registered in the aruana grass. Infective larvae (L3) recovery was similar among the different strata during spring. In springtime, the biggest L3 recovery occurred at the 21-28 cm stratum from both forage species. No L3 was recovered from any of the No L3 was recovered from any of the grass strata during winter and summer. Study results show that migration of T. colubriformis larvae was more influenced by weather conditions than by forage species.
该实验的目的是评估感染性蛇形毛圆线虫幼虫在两种饲草中的垂直迁移情况。本研究使用了由八个地块组成的实验模块,这些地块分别种植了臂形草品种澳大利亚臂形草(Brachiaria decumbens cv. Australian)和大黍品种阿鲁阿纳大黍(Panicum maximum cv. Aruana),每种草各有四个地块。每个地块被分成六个30×30厘米的子地块。在一年的四个季节里,对不同植物层(0 - 7厘米、7 - 14厘米、14 - 21厘米、21 - 28厘米及28厘米以上)的幼虫迁移情况进行评估。在一年的每个季节,在30厘米高的饲草中间放置四份粪便。在实验子地块每次放置粪便十天后,从饲草中收集粪便。在每次收集前,立即测量各层的草高。从半径为10厘米的区域切割不同层的饲草。通过人工从子地块收集粪便。秋季放置粪便时存在草种和草层的交互作用(P<0.05)。在那个季节,大部分幼虫是从臂形草基部回收的;与此同时,在饲草顶端,阿鲁阿纳草的平均回收量最大。春季不同层间感染性幼虫(L3)的回收情况相似。在春季,两种饲草在21 - 28厘米层的L3回收量最大。在冬季和夏季,从任何草层均未回收L3。研究结果表明,蛇形毛圆线虫幼虫的迁移受天气条件的影响大于饲草种类的影响。