Chiba N
Second Department of Internal Medicine, Hirosaki University School of Medicine.
Nihon Jinzo Gakkai Shi. 1991 Oct;33(10):925-38.
In order to clarify considerable alterations of the extracellular matrix components in various renal diseases, monoclonal antibodies against type IV collagen (IV col), 200-KD laminin (200-KD Lam), 400-KD laminin (400-KD Lam) and heparan sulfate proteoglycan (HSPG) were applied to 142 renal biopsy specimens for the indirect immunofluorescence. These subjects included 5 cases with 1 hour specimen in the transplanted kidney, 32 minimal change nephrotic syndrome (MCNS), 30 mesangial proliferative glomerulonephritis (PGN), 2 focal segmental glomerulosclerosis (FGS), 21 membranous nephropathy (MN), 9 membranoproliferative glomerulonephritis (MPGN), 2 poststreptococcal acute glomerulonephritis (PSAGN), 13 diabetic nephropathy (DN), 16 lupus nephritis (LN), 9 diffuse sclerosing glomerulonephritis (DSGN), 2 amyloid kidney and 1 granulomatous nephropathy in sarcoidosis. In the transplanted kidney, the staining intensity of IV col was stronger than that of 200-KD Lam, 400-KD Lam and HSPG in general. IV col was predominantly distributed throughout the mesangium area and less along the glomerular basement membrane (GBM). The positive stainings with 200-KD Lam along the GBM and that with 400-KD Lam in the mesangium area were weakly recognized. HSPG was mainly detected along the GBM in the linear fashion. In MCNS, the distribution of the extracellular matrix components was mostly identical to that in the transplanted kidney. In the group of the glomerulonephritis showed the proliferations of mesangial cells, such as PGN and MPGN, the staining intensity of both IV col and 400-KD Lam, particularly in the latter, was remarkably increased in the sclerotic lesions. In MN, the thickened GBM was strongly stained with both IV col and 200-KD Lam, and the stainings of 200-KD Lam were more intensive. And still more, by the double labelling method performed for the couple of IV col and immunoglobulins, the correlation between glomerular capillary walls and/or mesangial areas and immunoglobulins deposits became more clear. These findings suggest as follows: (1) both IV col and 400-KD Lam, in particular 400-KD Lam, are possibly involved in the process of glomerulosclerosis: (2) both IV col and 200-KD Lam, in particular 200-KD Lam, are greatly involved in the process of new basement membrane-like materials formation in MN.
为阐明各种肾脏疾病中细胞外基质成分的显著改变，将抗IV型胶原（IV col）、200-KD层粘连蛋白（200-KD Lam）、400-KD层粘连蛋白（400-KD Lam）和硫酸乙酰肝素蛋白聚糖（HSPG）的单克隆抗体应用于142份肾活检标本进行间接免疫荧光检测。这些受试者包括5例移植肾1小时标本、32例微小病变肾病（MCNS）、30例系膜增生性肾小球肾炎（PGN）、2例局灶节段性肾小球硬化（FGS）、21例膜性肾病（MN）、9例膜增生性肾小球肾炎（MPGN）、2例链球菌感染后急性肾小球肾炎（PSAGN）、13例糖尿病肾病（DN）、16例狼疮性肾炎（LN）、9例弥漫性硬化性肾小球肾炎（DSGN）、2例淀粉样肾病和1例结节病肉芽肿性肾病。在移植肾中，IV col的染色强度总体上强于200-KD Lam、400-KD Lam和HSPG。IV col主要分布于整个系膜区，沿肾小球基底膜（GBM）分布较少。沿GBM的200-KD Lam阳性染色及系膜区的400-KD Lam阳性染色较弱。HSPG主要沿GBM呈线性方式检测到。在MCNS中，细胞外基质成分的分布与移植肾中的情况大多相同。在系膜细胞增生的肾小球肾炎组，如PGN和MPGN，IV col和400-KD Lam的染色强度，尤其是后者，在硬化病变中显著增加。在MN中，增厚的GBM被IV col和200-KD Lam强烈染色，且200-KD Lam的染色更强烈。此外，通过对IV col和免疫球蛋白进行双重标记法，肾小球毛细血管壁和/或系膜区与免疫球蛋白沉积之间的相关性变得更加清晰。这些发现提示如下：（1）IV col和400-KD Lam，尤其是400-KD Lam，可能参与肾小球硬化过程；（2）IV col和200-KD Lam，尤其是200-KD Lam，在MN中新基底膜样物质形成过程中起重要作用。
