Kutateladze Tatiana G
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA.
Prog Lipid Res. 2007 Nov;46(6):315-27. doi: 10.1016/j.plipres.2007.06.001. Epub 2007 Jul 13.
Phosphatidylinositol 3-phosphate [PtdIns(3)P], a phospholipid produced by PI 3-kinases in early endosomes and multivesicular bodies, often serves as a marker of endosomal membranes. PtdIns(3)P recruits and activates effector proteins containing the FYVE or PX domain and therefore regulates a variety of biological processes including endo- and exocytosis, membrane trafficking, protein sorting, signal transduction and cytoskeletal rearrangement. Structures and PtdIns(3)P binding modes of several FYVE and PX domains have recently been characterized, unveiling the molecular basis underlying multiple cellular functions of these proteins. Here, structural and functional aspects and current mechanisms of the multivalent membrane anchoring by the FYVE and PX domains are reviewed and compared.
磷脂酰肌醇3-磷酸[PtdIns(3)P]是一种由PI 3激酶在早期内体和多囊泡体中产生的磷脂,常作为内体膜的标志物。PtdIns(3)P招募并激活含有FYVE或PX结构域的效应蛋白,从而调节包括胞吞和胞吐、膜运输、蛋白质分选、信号转导和细胞骨架重排在内的多种生物学过程。最近已对几种FYVE和PX结构域的结构及PtdIns(3)P结合模式进行了表征,揭示了这些蛋白多种细胞功能的分子基础。本文对FYVE和PX结构域多价膜锚定的结构和功能方面以及当前机制进行了综述和比较。