Chen Li-Juan, Li Jian-Yong, Wu Yu-Jie, Yang Hui, Qian Si-Xuan, Wu Han-Xin, Lu Hua, Xu Wei, Sheng Rui-Lan
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Aug;15(4):692-5.
The objective of this study was to investigate the immunophenotypic characteristics of T-cell acute lymphoblastic leukemia (T-ALL). Immunophenotyping was performed in 140 T-ALL patients by flow cytometry using a panel of monoclonal antibodies and CD45/SSC gating. The results showed that the T-lineage-associated antigen expressions were CD7 > CD2 > CD3 > CD5 successively. The positive rate of CD10 was 19.42% in patients. Among 140 cases of T-ALL, 12 (8.57%) was accompanied by B-lineage associated antigen expression. Myeloid antigen expression was identified in 31 out of 136 cases (22.79%). None of them expressed CD14 antigen. The positive rate of CD34 was 31.06%. The positive rate of myeloid antigen expression in CD34(+) T-ALL (36.58%) was significantly higher than that in CD34(-) T-ALL (15.38%) (p < 0.01). The expression of CD3 in child T-ALL was higher than that in adult T-ALL, whereas the expression of CD33 in children was lower than that in adults. It is concluded that immunophenotyping is an important tool for diagnosis of T-ALL. Immunophenotypic characteristics of T-ALL is heterogeneous.
本研究的目的是调查T细胞急性淋巴细胞白血病（T-ALL）的免疫表型特征。采用一组单克隆抗体和CD45/SSC设门，通过流式细胞术对140例T-ALL患者进行免疫表型分析。结果显示，T系相关抗原表达依次为CD7＞CD2＞CD3＞CD5。患者中CD10阳性率为19.42%。在140例T-ALL病例中，12例（8.57%）伴有B系相关抗原表达。136例中有31例（22.79%）检测到髓系抗原表达。均未表达CD14抗原。CD34阳性率为31.06%。CD34(+)T-ALL中髓系抗原表达阳性率（36.58%）显著高于CD34(-)T-ALL（15.38%）（p＜0.01）。儿童T-ALL中CD3的表达高于成人T-ALL，而儿童CD33的表达低于成人。结论是免疫表型分析是诊断T-ALL的重要工具。T-ALL的免疫表型特征具有异质性。
