Sun Qian, Chen Bao-An, Wang Xue-Mei, Gao Feng, Dai Yong-Yuan, Cheng Jian, Ding Jia-Hua, Gao Chong, Li Jing-Yuan, Xu Wen-Lin, Shen Hui-Ling
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Aug;15(4):748-51.
To prepare Fe(3)O(4)-magnetic nanoparticles loaded with adriamycin and investigate the reversal role of drug-loaded nanoparticles in K562 and resistant cell line K562/A02, the drug-loaded nanoparticles were prepared by using mechanical absorption polymerization at different conditions of 4 degrees C or 37 degrees C for 24 or 48 hours. The survival of cells cultured with drug-loaded nanoparticles for 48 hours was detected by MTT assay, then the growth inhibition efficacy of cells was calculated. The results showed that the growth inhibition efficacy of both two cell lines was enhanced with increasing concentration of Fe(3)O(4)-magnetic nanoparticles. The inhibitory ratio of two cell lines obtained at 4 degrees C and for 48 hours was significantly better than that at 37 degrees C and 24 hours. In conclusion, Fe(3)O(4)-magnetic nanoparticles can load adriamycin by using mechanical absorption polymerization, but depended on proper temperature and time. Furthermore, drug-loaded nanoparticles showed an ability reversing multidrug resistance.
制备负载阿霉素的Fe(3)O(4)磁性纳米颗粒，并研究载药纳米颗粒对K562及其耐药细胞株K562/A02的逆转作用。通过机械吸附聚合法在4℃或37℃不同条件下制备载药纳米颗粒24小时或48小时。采用MTT法检测用载药纳米颗粒培养48小时后细胞的存活率，进而计算细胞的生长抑制率。结果显示，两种细胞系的生长抑制率均随Fe(3)O(4)磁性纳米颗粒浓度的增加而增强。在4℃下制备48小时获得的两种细胞系的抑制率显著优于在37℃下制备24小时的抑制率。总之，Fe(3)O(4)磁性纳米颗粒可通过机械吸附聚合法负载阿霉素，但这取决于合适的温度和时间。此外，载药纳米颗粒显示出逆转多药耐药的能力。
