Liao Bin, Ge Ren-Ying, Chen Xia, Huangfu Zhen-Ping, Qi Yan, Song Yong-Ping, Wei Xu-Dong
Department of Hematology, The People Hospital of Fujian Province, Fujian Traditional Chinese Medical College, Fuzhou 350004, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Aug;15(4):752-5.
The study was purposed to investigate the synergistic reversal effect of Chinese medicine compound FFJZ in combination with cyclosporine A (CsA) on the multidrug resistance (MDR) of human leukemia K562/VCR cell line, as to search effective combination of MDR modulators. MTT (methyl-thazol-tetrazolinum) assay were used to determine the cytotoic and reversal effects on K562/VCR cell line, FCM (flow cytometry) was used to assess the intracellular adriamycin (ADM) concentration and the expression of P-gp in cells. The results showed that the FFJZ in combination with CsA could reverse the drug-resistance of K562/VCR cells and increase the sensitivity K562/VCR cells to adriamycin. They had not the toxic effect on the K562/VCR cells in effective dose and no significant influence on P-gp positive rate of the K562/VCR cells. It is concluded that the FFJZ in combination with CsA may become a safe and effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy.
本研究旨在探讨中药复方FFJZ联合环孢素A（CsA）对人白血病K562/VCR细胞株多药耐药（MDR）的协同逆转作用，以寻找有效的多药耐药调节剂组合。采用MTT（甲基噻唑四唑蓝）法检测其对K562/VCR细胞株的细胞毒性及逆转作用，应用流式细胞术（FCM）检测细胞内阿霉素（ADM）浓度及P-糖蛋白（P-gp）的表达。结果显示，FFJZ联合CsA可逆转K562/VCR细胞的耐药性，增加其对阿霉素的敏感性。在有效剂量下对K562/VCR细胞无毒性作用，对K562/VCR细胞的P-gp阳性率无显著影响。结论：FFJZ联合CsA可能成为白血病化疗中一种安全有效的低毒性多药耐药逆转剂。
