[重组葡激酶对大鼠重症急性胰腺炎胰腺缺血的影响]
[Effect of the recombinant staphylokinase on pancreatic ischemia in severe acute pancreatitis of rats].
作者信息
Sha Jian-ping, Xue Yao-ming, Chen Xuan, Zhu Bi-de, Zhang Xin-sheng, Chen Wei-yin, Feng Ben-hua, Zhao Yan
机构信息
Department of Endocrinology, the Southern Medical University, Guangzhou 510515, Guangdong, China.
出版信息
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Aug;19(8):467-70.
OBJECTIVE
To investigate the changes in plasma endothelin-1 (ET-1) , von Willebrand factor (vWF), serum 6-keto-prostaglandin(1alpha) (PGF(1alpha)) , thromboxane B2 (TXB2), platelet aggregation rate maximum (PAGm) and pancreatic blood flow after reproduction of severe acute pancreatitis (SAP) in rat, and the effect of recombinant staphylokinase (r-Sak) on SAP.
METHODS
Eighty-one SD rats were divided randomly into the sham-operated group (n=27), the SAP model group (n=27), and the r-Sak treatment group (n=27). SAP was produced by administration of 5% sodium taurocholate into the pancreatic duct. The abdomen of rats was opened at 6, 12 and 18 hours after reproduction of SAP for determining the pancreatic blood flow. Blood was obtained at 6, 12 and 18 hours after reproduction of SAP for determining the concentration of plasma vWF with enzyme-labeled immunosorbent assay (ELISA). The concentration of plasma ET-1 and serum 6-keto-PGF(1alpha), and TXB2 were detected by radioimmunoassay. The PAGm induced by collagen and eicosanoids was assessed.
RESULTS
Pancreatic blood flow in the SAP group appeared to have a decreasing trend at 6,12 and 18 hours after operation and were significantly decreased at all time points after reproduction of the model, compared with those of the sham-operated group (all P<0.05). The PAGm, content of plasma ET-1, vWF, and TXB2 were significantly increased at all time points after reproduction of the model, while 6-keto-PGF(1alpha) was significantly decreased, compared with those of the sham-operated group (all P < 0.05). Compared with SAP model group, PAGm, the content of plasma ET-1, vWF, and serum TXB2 in the r-Sak group were decreased at all time points, however, the content of serum 6-keto-PGF(1alpha) was increased (all P<0.05).
CONCLUSION
The r-Sak can improve pancreatic microcirculation and enhance pancreatic blood flow in rats with SAP, and may be beneficial in the treatment of SAP.
目的
探讨大鼠重症急性胰腺炎（SAP）造模后血浆内皮素-1（ET-1）、血管性血友病因子（vWF）、血清6-酮-前列腺素（1α）（PGF（1α））、血栓素B2（TXB2）、血小板最大聚集率（PAGm）及胰腺血流量的变化，以及重组葡激酶（r-Sak）对SAP的影响。
方法
将81只SD大鼠随机分为假手术组（n = 27）、SAP模型组（n = 27）和r-Sak治疗组（n = 27）。通过向胰管内注入5%牛磺胆酸钠制备SAP模型。在SAP造模后6、12和18小时打开大鼠腹部测定胰腺血流量。在SAP造模后6、12和18小时取血，采用酶联免疫吸附测定法（ELISA）测定血浆vWF浓度。采用放射免疫法检测血浆ET-1、血清6-酮-PGF（1α）和TXB2的浓度。评估胶原和类花生酸诱导的PAGm。
结果
与假手术组相比，SAP组术后6、12和18小时胰腺血流量呈下降趋势，造模后各时间点均显著降低（均P < 0.05）。与假手术组相比，造模后各时间点PAGm、血浆ET-1、vWF和TXB2含量显著升高，而6-酮-PGF（1α）显著降低（均P < 0.05）。与SAP模型组相比，r-Sak组各时间点PAGm、血浆ET-1、vWF和血清TXB2含量均降低，而血清6-酮-PGF（1α）含量升高（均P < 0.05）。
结论
r-Sak可改善SAP大鼠的胰腺微循环，增加胰腺血流量，可能对SAP治疗有益。
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