大鼠重症急性胰腺炎早期肺损伤的实验研究

[Experimental study on early pulmonary injury induced by severe acute pancreatitis in rats].

作者信息

Zhang Yi-sheng, Wang Ming-hai, Tao Kun, Zhao Guo-hai

机构信息

Department of Surgical Emergency, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, China.

出版信息

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Dec;19(12):753-5.

PMID:18093438

Abstract

OBJECTIVE

To investigate the injury to pulmonary tissue induced by severe acute pancreatitis (SAP) during its early period.

METHODS

Forty adult male Sprague-Dawley (SD) rats weighing 300-400 g were randomly divided into 4 groups with 8 animals in each group: sham operation group and 30, 60, 120 and 360 minutes after SAP groups. The model of SAP was reproduced by retrograde injection of 5% sodium taurocholate into the pancreatic duct of rats. Rats were sacrificed at different time points, and blood gas analysis, pulmonary pathological changes and wet dry weight ratio (W/D) of the lung were conducted.

RESULTS

A large amount of polymorphonuclear neutrophils were observed in pulmonary tissue within 360 minutes after reproduction of SAP with obvious changes, and water content of pulmonary tissue was increased significantly, while base excess (BE) decreased significantly in rats with SAP (P<0.05 or P<0.01 ). But no obvious change was observed in arterial partial pressure of oxygen (PaO(2)) and partial pressure of carbon dioxide (PaCO(2), both P>0.05).

CONCLUSION

SAP causes significant inflammation in rat lung with injury to pulmonary tissue in its early period.

摘要

目的

探讨重症急性胰腺炎（SAP）早期对肺组织的损伤。

方法

将40只体重300 - 400 g的成年雄性Sprague-Dawley（SD）大鼠随机分为4组，每组8只：假手术组以及SAP术后30、60、120和360分钟组。通过向大鼠胰管逆行注射5%牛磺胆酸钠复制SAP模型。在不同时间点处死大鼠，进行血气分析、肺组织病理学变化及肺组织湿干重比（W/D）测定。