The Dept of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Trends Cell Biol. 1997 Sep;7(9):368-73. doi: 10.1016/S0962-8924(97)01113-6.
Nematodes produce amoeboid sperm that crawl over surfaces in a manner reminiscent of many actin-rich cells. However, these sperm contain no F-actin, and their motility is powered by a dynamic filament system composed of polymers of the 14-kDa major sperm protein (MSP). These simple cells use this unique motility apparatus exclusively for locomotion. Recent studies have capitalized on this feature to explore the key structural properties of MSP related to its role in motility and to reconstitute the motility apparatus both in vivo and in vitro. This review discusses how these investigations have laid the foundation for understanding the physical basis of amoeboid movement by identifying the mechanistic properties shared by the MSP-based machinery and the more familiar actin-based systems.
线虫产生变形虫状的精子，这些精子在表面爬行的方式让人联想到许多富含肌动蛋白的细胞。然而，这些精子不含 F-肌动蛋白，它们的运动是由由 14kDa 主要精子蛋白（MSP）的聚合物组成的动态细丝系统提供动力的。这些简单的细胞专门使用这种独特的运动装置进行运动。最近的研究利用这一特点来探索与 MSP 运动功能相关的关键结构特性，并在体内和体外重建运动装置。本文综述了这些研究如何通过确定基于 MSP 的机制与更熟悉的基于肌动蛋白的系统所共有的力学特性，为理解变形虫运动的物理基础奠定了基础。
