Siejak Przemysław, Frackowiak Danuta
Institute of Physics, Poznań University of Technology, Nieszawska 13A, 60-965 Poznań, Poland.
J Photochem Photobiol B. 2007 Sep 25;88(2-3):126-30. doi: 10.1016/j.jphotobiol.2007.06.001. Epub 2007 Jul 4.
Changes in the yield of the fluorescence emitted by pigments of photosynthetic organisms could be used for the establishment of the presence of some toxic substances. The presence of colloidal metals can be indicated by enhancement of pigments' emission as a result of plasmons generation. The spectra of the pigments of cyanobacterium Synechocystis located in the bacterium fragments and in solutions with and without colloidal silver additions have been measured. The quantum yield of the pigments' fluorescence in solution has been observed to increase at some wavelength of excitation, while the fluorescence of the pigments in the bacteria fragments has been only quenched as a consequence of interactions with colloidal silver particles. Close contact between pigment molecules located in bacteria fragments and silver particles is probably not possible. We plan in future to investigate the influence of other, more typical metal pollutants of water, using similar spectral methods and several other photosynthetic bacteria pigments, in solution, in cell fragments and in the whole bacteria organisms.
光合生物色素发出的荧光产量变化可用于确定某些有毒物质的存在。由于等离子体激元的产生，色素发射增强可表明胶体金属的存在。已测量了位于细菌碎片中以及添加和未添加胶体银的溶液中的蓝藻聚球藻色素光谱。已观察到溶液中色素荧光的量子产率在某些激发波长下会增加，而细菌碎片中色素的荧光仅因与胶体银颗粒的相互作用而猝灭。位于细菌碎片中的色素分子与银颗粒之间可能无法紧密接触。我们计划未来使用类似的光谱方法以及溶液、细胞碎片和整个细菌生物体中的其他几种光合细菌色素，研究其他更典型的水中金属污染物的影响。
