Ferrier Moira C, Sarin Hemant, Fung Steve H, Schatlo Bawarjan, Pluta Ryszard M, Gupta Sandeep N, Choyke Peter L, Oldfield Edward H, Thomasson David, Butman John A
Diagnostic Radiology Department, Imaging Sciences Program, The Clinical Center of the National Institutes of Health, Bethesda, MD 20892, USA.
Neoplasia. 2007 Jul;9(7):546-55. doi: 10.1593/neo.07289.
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is widely used to evaluate tumor permeability, yet measurements have not been directly validated in brain tumors. Our purpose was to compare estimates of forward leakage K(trans) derived from DCE-MRI to the estimates K obtained using [(14)C]aminoisobutyric acid quantitative autoradiography ([(14)C]AIB QAR), an established method of evaluating blood-tumor barrier permeability. Both DCE-MRI and [(14)C]AIB QAR were performed in five rats 9 to 11 days following tumor implantation. K(trans) in the tumor was estimated from DCE-MRI using the threeparameter general kinetic model and a measured vascular input function. K(i) was estimated from QAR data using regions of interest (ROI) closely corresponding to those used to estimate K(trans). K(trans) and K(i) correlated with each other for two independent sets of central tumor ROI (R = 0.905, P = .035; R = 0.933, P = .021). In an additional six rats, K(trans) was estimated on two occasions to show reproducibility (intraclass coefficient = 0.9993; coefficient of variance = 6.07%). In vivo blood-tumor permeability parameters derived from DCE-MRI are reproducible and correlate with the gold standard for quantifying blood tumor barrier permeability, [(14)C]AIB QAR.
动态对比增强磁共振成像（DCE-MRI）被广泛用于评估肿瘤通透性，但相关测量在脑肿瘤中尚未得到直接验证。我们的目的是比较DCE-MRI得出的正向渗漏K（trans）估计值与使用[（14）C]氨基异丁酸定量放射自显影术（[（14）C]AIB QAR）获得的K估计值，[（14）C]AIB QAR是一种评估血脑屏障通透性的成熟方法。在肿瘤植入后9至11天对5只大鼠进行了DCE-MRI和[（14）C]AIB QAR检查。使用三参数通用动力学模型和测量的血管输入函数从DCE-MRI估计肿瘤中的K（trans）。使用与用于估计K（trans）的区域密切对应的感兴趣区域（ROI）从QAR数据估计K（i）。两组独立的肿瘤中心ROI的K（trans）和K（i）相互相关（R = 0.905，P = 0.035；R = 0.933，P = 0.021）。在另外6只大鼠中，两次估计K（trans）以显示其可重复性（组内系数 = 0.9993；变异系数 = 6.07%）。从DCE-MRI得出的体内血瘤通透性参数具有可重复性，并且与量化血脑屏障通透性的金标准[（14）C]AIB QAR相关。
