Korkmaz Ahmet, Oter Sükrü, Sadir Serdar, Topal Turgut, Uysal Bülent, Ozler Mehmet, Ay Hakan, Akin Ahmet
Department of Physiology, Gülhane Askeri Tip Akademisi, Fizyoloji Anabilim Dali, 06018 Ankara, Turkey.
Neurochem Res. 2008 Jan;33(1):160-6. doi: 10.1007/s11064-007-9428-4. Epub 2007 Aug 21.
Hyperbaric oxygen (HBO) is known to cause oxidative stress in several organs and tissues. Due to its high rate of blood flow and oxygen consumption, the brain is one of the most sensitive organs to this effect. The present study was performed to elucidate the relation of HBO exposure time to its oxidative effects in rats' brain cortex tissue. For this purpose, 49 rats were randomly divided into five groups. Except the control group, study groups were subjected to three atmospheres HBO for 30, 60, 90, and 120 min. Their cerebral cortex layer was taken immediately after exposure and used for analysis. Thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and nitrate-nitrite (NOX) levels were determined. TBARS and SOD levels were found to increase in a time-dependent manner. GSH-Px activity reflected an inconsistent course. NOX levels were found to be increased only in the 120 min exposed group. The results of this study suggests that HBO induced oxidative effects are strongly related with exposure time.
已知高压氧（HBO）会在多个器官和组织中引发氧化应激。由于大脑的血流速度和氧耗率较高，它是对这种影响最为敏感的器官之一。本研究旨在阐明HBO暴露时间与其对大鼠大脑皮质组织氧化作用之间的关系。为此，将49只大鼠随机分为五组。除对照组外，研究组接受三个大气压的HBO处理30、60、90和120分钟。暴露后立即取出它们的大脑皮质层用于分析。测定了硫代巴比妥酸反应性物质（TBARS）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和硝酸盐-亚硝酸盐（NOX）水平。发现TBARS和SOD水平呈时间依赖性增加。GSH-Px活性呈现出不一致的变化过程。仅在暴露120分钟的组中发现NOX水平升高。本研究结果表明，HBO诱导的氧化作用与暴露时间密切相关。
Zotero 插件