Suppr超能文献

一种口服有效的超氧化物歧化酶对高压氧相关细胞损伤的影响。

Influence of an orally effective SOD on hyperbaric oxygen-related cell damage.

作者信息

Muth Claus M, Glenz Yvonne, Klaus Michael, Radermacher Peter, Speit Günter, Leverve Xavier

机构信息

Sektion Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Universitätsklinikum Ulm, D-89073 Ulm, Germany.

出版信息

Free Radic Res. 2004 Sep;38(9):927-32. doi: 10.1080/10715760412331273197.

Abstract

In a prospective, double-blind, randomised placebo-controlled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress. Twenty healthy volunteers were exposed to 100% oxygen breathing at 2.5 ATA for a total of 60 min. DNA strand breaks (tail moments) were determined using the alkaline version of the comet assay. Whole blood concentrations of reduced (GSH) and oxidised (GSSG) glutathione and F2-isoprostanes, SOD, glutathione peroxidase (GPx) and catalase (Cat) activities and red cell malondialdehyde (MDA) content were determined. After HBO exposure the tail moment (p = 0.03) and isoprostane levels (p = 0.049) were significantly lower in the group that received the vegetal formulation. Neither SOD and Cat nor GSH and GSSG were significantly affected by this preparation or HBO exposure. By contrast, blood GPx activity, which tended to be lower in the SOD-group already before the HBO exposure (p = 0.076), was significantly lower afterwards (p = 0.045). We conclude that an orally effective SOD-wheat gliadin mixture is able to protect against DNA damage, which coincided with reduced blood isoprostane levels, and may therefore be used as an antioxidant.

摘要

在一项前瞻性、双盲、随机、安慰剂对照研究中,我们检验了以下假设:一种由小麦醇溶蛋白与植物性(因此口服有效)超氧化物歧化酶(SOD)化学结合而成的新制剂能够预防高压氧(HBO)诱导的氧化细胞应激。20名健康志愿者在2.5ATA下呼吸100%氧气,共60分钟。使用彗星试验的碱性版本测定DNA链断裂(尾矩)。测定全血中还原型(GSH)和氧化型(GSSG)谷胱甘肽、F2-异前列腺素、SOD、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(Cat)的活性以及红细胞丙二醛(MDA)含量。HBO暴露后,接受植物性制剂的组尾矩(p = 0.03)和异前列腺素水平(p = 0.049)显著降低。该制剂或HBO暴露对SOD和Cat以及GSH和GSSG均无显著影响。相比之下,在HBO暴露前SOD组中就已趋于降低的血液GPx活性(p = 0.076),暴露后显著降低(p = 0.045)。我们得出结论,口服有效的SOD-小麦醇溶蛋白混合物能够预防DNA损伤,这与血液中异前列腺素水平降低相一致,因此可用作抗氧化剂。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验