Ye Wei, Ma Ruo-Fan, Ding Yue, Huang Dong-Sheng, Chen Wei-Jian, Peng Yan, Liu Shang-Li
Department of Orthopedics, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Aug;27(8):1187-9.
To evaluate the effect of interleukin-6 (IL-6) on the biological behaviors of the chondrocytes in the cartilage endplate of rabbits.
Chondrocytes isolated from the cartilage endplate of New Zealand rabbits, verified for their biological characteristics by such means as toluidine blue staining for type II collagen, were treated with IL-6 at different concentrations. The proliferation of the chondrocytes was evaluated by MTT assay at different time points following the treatment, the cell cycle changes were determined by flow cytometry and the changes of aggrecan and type II collagen mRNAs detected by RT-PCR.
At the concentrations of 10, 50 and 100 ng/ml, IL-6 did not obviously affect the rate of chondrocyte proliferation. IL-6 at 50 ng/ml resulted in no obvious changes of the cell cycle of the chondrocytes, but significantly decreased the expression of collagen IIa mRNA.
IL-6 has no effect on the proliferation and cell cycle of the chondrocytes, but at higher concentrations, it inhibits matrix synthesis of the chondrocytes to promote intervertebral disc degeneration.
评估白细胞介素-6（IL-6）对兔软骨终板软骨细胞生物学行为的影响。
从新西兰兔软骨终板分离软骨细胞，通过II型胶原甲苯胺蓝染色等方法验证其生物学特性，用不同浓度的IL-6处理。处理后不同时间点通过MTT法评估软骨细胞增殖，通过流式细胞术测定细胞周期变化，通过RT-PCR检测聚集蛋白聚糖和II型胶原mRNA的变化。
在10、50和100 ng/ml浓度下，IL-6对软骨细胞增殖率无明显影响。50 ng/ml的IL-6对软骨细胞的细胞周期无明显改变，但显著降低了IIa型胶原mRNA的表达。
IL-6对软骨细胞的增殖和细胞周期无影响，但在较高浓度时，它抑制软骨细胞的基质合成以促进椎间盘退变。
