Chen Jing-hong, Cao Jun-ling, Chu Yong-lie, Yang Zhan-tian, Shi Zhong-li, Wang Hong-lin, Guo Xiong, Wang Zhi-lun
Institute of Endemic Diseases, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Apr;26(4):381-5.
To study the inhibitory effect of T-2 toxin on the expression of aggrecan and collagen II in chondrocytes and the protection of selenium against this effect.
Human chondrocytes cultured in vitro were treated with T-2 toxin at different concentrations for varied time periods (1-5 days), and the cell viability was measured by MTT assay. Aggrecan expression was detected by toluidine blue staining and collagen II expression by immunostaining using monoclonal antibody of collagen. Aggrecan and collagen II mRNA expressions were measured by semiquantitative RT-PCR.
T-2 toxin dose- and time-dependently affected chondrocyte viability within the concentration range of 0.001-2 mg/L, the prolonged treatment time further enhanced the dose dependence of the inhibitory effect. T-2 toxin lowered aggrecan and collagen II synthesis in the chondrocytes and reduced their mRNA expressions. Selenium could partly attenuate the inhibitory effects of T-2 toxin on aggrecan mRNA expression, but showed no such effect against T-2-induced collagen II expression.
T-2 toxin can obviously inhibit aggrecan and collagen II synthesis in human chondrocytes, and selenium can partly antagonize the inhibitory effects of T-2 toxin on aggrecan.
研究T-2毒素对软骨细胞中聚集蛋白聚糖和Ⅱ型胶原蛋白表达的抑制作用以及硒对该作用的保护效应。
体外培养的人软骨细胞用不同浓度的T-2毒素处理不同时间（1 - 5天），采用MTT法检测细胞活力。用甲苯胺蓝染色检测聚集蛋白聚糖表达，用Ⅱ型胶原蛋白单克隆抗体免疫染色检测Ⅱ型胶原蛋白表达。采用半定量RT-PCR检测聚集蛋白聚糖和Ⅱ型胶原蛋白mRNA表达。
在0.001 - 2 mg/L浓度范围内，T-2毒素对软骨细胞活力的影响呈剂量和时间依赖性，延长处理时间进一步增强了抑制作用的剂量依赖性。T-2毒素降低了软骨细胞中聚集蛋白聚糖和Ⅱ型胶原蛋白的合成，并降低了它们的mRNA表达。硒可部分减弱T-2毒素对聚集蛋白聚糖mRNA表达的抑制作用，但对T-2毒素诱导的Ⅱ型胶原蛋白表达无此作用。
T-2毒素可明显抑制人软骨细胞中聚集蛋白聚糖和Ⅱ型胶原蛋白的合成，硒可部分拮抗T-2毒素对聚集蛋白聚糖的抑制作用。
