Liang Wei-Jiang, Yan Xi, Zhang Wan-Dai, Luo Rong-Cheng
Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Aug;27(8):1241-3.
To explore the inhibitory effect of garlic oil on cyclin E expression in gastric adenocarcinoma cells.
Human gastric adenocarcinoma SGC7901 cells were cultured routinely and the expressions of transforming growth factor alpha (TGFalpha) and epidermal growth factor receptor (EGFR) are detected by immunofluorescent staining and flow cytometry. The SGC7901 cells were also cultured with RPMI 1640 without calf serum for 48 h, followed by further culture with RPMI 1640 in the presence of 2.5% calf serum before treatment with TGFalpha, garlic oil, or their combination, and cyclin E expression of the cells was then detected by immunofluorescent staining and flow cytometry.
The positivity rates of TGFalpha and EGFR expressions were 46.80% and 57.78 % respectively in SGC7901 cells cultured routinely for 48 h. The positivity rate of cyclin E expression was increased by 7.06% (P<0.001) in SGC7901 cells treated with 30 microg/L TGFalpha for 5 h, decreased by 11.75% (P<0.001) following a 5-hour treatment with 10% garlic oil, and decreased further by 17.11% (Plt;0.001) after treatment with both 30 microg/L TGFalpha and 10% garlic oil for 5 h.
The gastric adenocarcinoma SGC7901 cells express TGFalpha and EGFR and possess TGFalpha autocrine and paracrine loops to promote cell proliferation. Garlic oil inhibits cyclin E expression in routinely cultured SGC7901 cells and also in TGFalpha-treated ones, suggesting that garlic oil can inhibit the TGFalpha autocrine and paracrine loops, which can be one of the pathways of garlic oil to inhibit cancer cell proliferation.
探讨大蒜油对胃腺癌细胞中细胞周期蛋白E表达的抑制作用。
常规培养人胃腺癌SGC7901细胞，采用免疫荧光染色和流式细胞术检测转化生长因子α（TGFα）和表皮生长因子受体（EGFR）的表达。将SGC7901细胞也用不含小牛血清的RPMI 1640培养基培养48小时，然后在2.5%小牛血清存在下用RPMI 1640进一步培养，再用TGFα、大蒜油或它们的组合进行处理，随后通过免疫荧光染色和流式细胞术检测细胞的细胞周期蛋白E表达。
常规培养48小时的SGC7901细胞中，TGFα和EGFR表达的阳性率分别为46.80%和57.78%。用30μg/L TGFα处理5小时的SGC7901细胞中，细胞周期蛋白E表达的阳性率增加了7.06%（P<0.001），用10%大蒜油处理5小时后降低了11.75%（P<0.001），用30μg/L TGFα和10%大蒜油共同处理5小时后进一步降低了17.11%（P<0.001）。
胃腺癌SGC7901细胞表达TGFα和EGFR，并具有TGFα自分泌和旁分泌环以促进细胞增殖。大蒜油抑制常规培养的SGC7901细胞以及TGFα处理的细胞中的细胞周期蛋白E表达，提示大蒜油可抑制TGFα自分泌和旁分泌环，这可能是大蒜油抑制癌细胞增殖的途径之一。
