Jaiswal Jyoti K, Fix Marina, Takano Takahiro, Nedergaard Maiken, Simon Sanford M
The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA.
Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14151-6. doi: 10.1073/pnas.0704935104. Epub 2007 Aug 21.
Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used acridine orange (AO) to label vesicles. Although AO is often used for imaging exocytosis, we found that imaging vesicles labeled with AO can result in their photolysis. Here, we define experimental and analytical approaches that permit us to distinguish unambiguously between fusion, leakage, and lysis of individual vesicles. We have used this approach to demonstrate that lysosomes undergo calcium-triggered exocytosis in astrocytes.
对单个囊泡胞吐作用进行光学成像，正在为细胞分泌的机制和调节提供新的见解。为了研究星形胶质细胞中钙触发的分泌，我们使用吖啶橙（AO）标记囊泡。尽管AO常用于胞吐作用成像，但我们发现对用AO标记的囊泡进行成像会导致其光解。在这里，我们定义了实验和分析方法，使我们能够明确区分单个囊泡的融合、渗漏和裂解。我们已使用这种方法证明溶酶体在星形胶质细胞中会发生钙触发的胞吐作用。
