UVA和UVB光对斑马鱼胚胎的光生物学效应：具备光修复系统的证据
Photobiological effects of UVA and UVB light in zebrafish embryos: evidence for a competent photorepair system.
作者信息
Dong Qiaoxiang, Svoboda Kurt, Tiersch Terrence R, Monroe W Todd
机构信息
Department of Biological and Agricultural Engineering, 149 E.B. Doran Building, Louisiana State University and LSU AgCenter, Baton Rouge, LA 70803, USA.
出版信息
J Photochem Photobiol B. 2007 Sep 25;88(2-3):137-46. doi: 10.1016/j.jphotobiol.2007.07.002. Epub 2007 Jul 21.
The consequences of UVB and UVA irradiation on hatch rate, mortality, and malformation were studied in embryonic zebrafish (Danio rerio). The use of zebrafish embryos has expanded from traditional developmental models to diverse studies, including many techniques utilizing light exposure. To characterize useful indicators of photodamage, the responses and threshold limits of UV radiation as a function of embryonic stage and fish source were evaluated. Significant differences in UVB susceptibility were observed in embryos at 3, 6-7, 12, and 24h post-fertilization (hpf), with the 1000-cell stage (3 hpf) having greatest tolerance to UVB. Embryos derived from zebrafish raised in outdoor ponds were more tolerant to UVB than were embryos from laboratory-raised fish. Combinations of UVB and UVA exposure were used to confirm the presence of a competent photorepair system in zebrafish that could return otherwise malformed embryos to a normal phenotype. Overall, embryonic zebrafish had large tolerances (LD(50) of 850 J/cm(2)) to UVA, confirming their suitability for photoactivation and photorepair studies.
研究了紫外线B（UVB）和紫外线A（UVA）照射对斑马鱼（Danio rerio）胚胎孵化率、死亡率和畸形的影响。斑马鱼胚胎的应用已从传统的发育模型扩展到各种研究，包括许多利用光照的技术。为了确定光损伤的有用指标，评估了紫外线辐射作为胚胎发育阶段和鱼类来源函数的反应和阈值。在受精后3、6 - 7、12和24小时（hpf）的胚胎中观察到UVB敏感性的显著差异，1000细胞期（3 hpf）对UVB的耐受性最强。来自室外池塘饲养的斑马鱼的胚胎比实验室饲养的斑马鱼的胚胎对UVB更具耐受性。使用UVB和UVA联合照射来证实斑马鱼中存在一个有效的光修复系统，该系统可以使原本畸形的胚胎恢复到正常表型。总体而言，斑马鱼胚胎对UVA具有较大的耐受性（半数致死剂量为850 J/cm²），证实它们适用于光激活和光修复研究。
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